Human 4-Hydroxynonenal (HNE)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E16214h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人4-羟基壬烯酸(HNE)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HNE含量。人中性粒细胞弹性蛋白酶（HNE）是一种丝氨酸蛋白酶，存在于中性粒细胞嗜天青颗粒中。HNE与多种疾病如肺气肿、囊性纤维化等密切相关。研究机制主要围绕抑制其活性来开发药物，以减轻因HNE过度表达引发的炎症和组织损伤。试剂盒检测范围为39 pg/mL-2500 pg/mL，适用于基础科研中氧化应激相关疾病模型的机制研究、药物或天然产物的抗氧化功效评价，以及代谢组学中脂质过氧化程度的动态监测，为探索HNE在生理病理过程中的调控作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
HNE
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
39 pg/mL-2500 pg/mL
灵敏度：
19.5 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	89
	Range %	82-96
1:200	Average %	90
	Range %	85-96
1:400	Average %	85
	Range %	81-92
1:800	Average %	98
	Range %	91-104

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average
2500	0.175	0.170	0.173
1250	0.261	0.273	0.267
625	0.383	0.408	0.396
312.5	0.669	0.629	0.649
156.25	1.008	1.021	1.014
78	1.345	1.353	1.349
39	1.879	1.784	1.832
0	2.611	2.525	2.568