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产品描述：人高分子量脂联素(HMW Adiponectin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、尿液样本中的HMW Adiponectin含量。HMW Adiponectin，即高分子量脂联素，是一种由脂肪细胞分泌的蛋白质激素，参与脂肪代谢和胰岛素敏感性调节。其研究机制主要涉及调节血糖稳态、抗炎和抗动脉粥样硬化作用，与2型糖尿病风险密切相关。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于基础研究中对代谢相关疾病机制探索、药物干预效果评估及生物标志物筛选等场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：30 kDa adipocyte complement related protein ELISA Kit; 30 kDa adipocyte complement-related protein ELISA Kit; ACDC ELISA Kit; Acrp 30 ELISA Kit; ACRP30 ELISA Kit; ADIPO_HUMAN ELISA Kit; Adipocyte ELISA Kit; Adipocyte C1q and collagen domain containing protein ELISA Kit; Adipocyte complement related 30 kDa protein ELISA Kit; Adipocyte complement related protein of 30 kDa ELISA Kit; Adipocyte complement-related 30 kDa protein ELISA Kit; adipocyte-specific secretory protein ELISA Kit; Adiponectin ELISA Kit; Adiponectin precursor ELISA Kit; adiponectin; C1Q and collagen domain containing ELISA Kit; Adipoq ELISA Kit; Adipose most abundant gene transcript 1 ELISA Kit; Adipose most abundant gene transcript 1 protein ELISA Kit; Adipose specific collagen like factor ELISA Kit; ADIPQTL1 ELISA Kit; ADPN ELISA Kit; APM 1 ELISA Kit; apM-1 ELISA Kit; APM1 ELISA Kit; C1q and collagen domain-containing protein ELISA Kit; GBP 28 ELISA Kit; GBP28 ELISA Kit; Gelatin binding protein ELISA Kit; Gelatin binding protein 28 ELISA Kit; gelatin-binding protein 28 ELISA Kit; Gelatin-binding protein ELISA Kit; OTTHUMP00000210047 ELISA Kit
缩写：HMW Adiponectin
Uniprot No.：Q15848
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, urine
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HMW adiponectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 87 | ||||
| Range % | 84-91 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:800 | Average % | 101 | ||||
| Range % | 96-106 | |||||
回收率：
| The recovery of human HMW adiponectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-104 | ||||
| EDTA plasma (n=4) | 92 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.1692.1172.1432.0401001.6791.6581.6691.566501.2451.2311.2381.135250.8320.8690.8510.74812.50.5130.5470.5300.4276.250.3450.3670.3560.2533.120.2030.2120.2080.10500.1020.1040.103 | ||||||
货期：3-5 working days
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