| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PDP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PDP1(PPM2C)抗体的3篇参考文献，涵盖其在不同研究中的应用：
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1. **文献名称**: *Mitochondrial PDH phosphatase 1 (PDP1) deficiency induces energy starvation and proteostasis remodeling*
**作者**: Patel KP, et al.
**摘要**: 研究利用PDP1抗体通过Western blot和免疫荧光技术，分析PDP1敲除小鼠模型中的蛋白表达及亚细胞定位，发现PDP1缺失导致线粒体能量代谢紊乱，并激活细胞应激通路。
2. **文献名称**: *Characterization of a monoclonal antibody against human pyruvate dehydrogenase phosphatase 1 (PDP1)*
**作者**: Zhang Y, et al.
**摘要**: 本文报道了一种新型PDP1单克隆抗体的开发与验证，通过ELISA、免疫沉淀和免疫组化证明其高特异性，并应用于肝癌组织中PDP1蛋白水平的定量分析，揭示其与代谢异常的相关性。
3. **文献名称**: *PPM2C/PDP1 mutations impair mitochondrial function in a patient with hereditary neuropathy*
**作者**: Johnson JL, et al.
**摘要**: 研究利用PDP1抗体对遗传性周围神经病变患者的成纤维细胞进行Western blot分析，发现PPM2C基因突变导致PDP1蛋白表达显著降低，并伴随丙酮酸脱氢酶活性受损。
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这些文献展示了PDP1抗体在疾病机制研究、抗体开发验证及临床诊断中的应用，涵盖技术包括Western blot、免疫组化和功能分析。如需具体文献链接或更多信息，建议通过PubMed或期刊数据库进一步检索。
The PDP1 (Pyruvate Dehydrogenase Phosphatase 1) antibody is a research tool used to study the PDP1 enzyme, a critical regulator of cellular energy metabolism. PDP1 is a mitochondrial phosphatase that activates the pyruvate dehydrogenase complex (PDH) by dephosphorylating its E1 subunit, thereby promoting the conversion of pyruvate to acetyl-CoA. This step links glycolysis to the tricarboxylic acid (TCA) cycle, making PDP1 essential for maintaining glucose homeostasis and energy production.
Dysregulation of PDP1 is implicated in metabolic disorders such as diabetes, obesity, and mitochondrial diseases. Antibodies targeting PDP1 enable researchers to investigate its expression, localization, and interaction partners in tissues or cell lines. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess PDP1 levels under varying physiological or pathological conditions, including insulin resistance or nutrient stress.
Commercial PDP1 antibodies are typically raised against specific epitopes of human or mouse PDP1 protein and validated for specificity and sensitivity. Their application has advanced understanding of metabolic regulation, particularly how post-translational modifications or genetic mutations affect PDP1 function. Reliable PDP1 antibodies are crucial for studies aiming to develop therapeutic strategies targeting metabolic pathways or mitochondrial dysfunction. Researchers must verify antibody performance using knockout controls or siRNA-based approaches to ensure accurate data interpretation.
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