| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD2AP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CD2AP抗体的3篇参考文献及其摘要概括：
1. **"CD2-associated protein regulates glomerular epithelial cell apoptosis in proteinuric kidney disease"**
- **作者**：Kim JM, et al.
- **摘要**：研究利用CD2AP抗体发现CD2AP缺失导致肾小球足细胞凋亡，揭示了CD2AP在蛋白尿性肾病中的保护作用及与细胞骨架稳定的关联。
2. **"CD2AP interacts with the cytoskeletal adaptor protein vinculin to regulate podocyte adhesion"**
- **作者**：Lehtonen S, et al.
- **摘要**：通过CD2AP抗体免疫共沉淀实验，证实CD2AP与vinculin的相互作用对足细胞黏附至关重要，为肾病综合征的分子机制提供了新见解。
3. **"Amyloid-β precursor protein (APP) binds to CD2AP and regulates synaptic function"**
- **作者**：Hoe HS, et al.
- **摘要**：利用CD2AP抗体发现CD2AP与APP的结合影响突触功能，提示其在阿尔茨海默病中可能通过调控APP代谢途径参与病理过程。
4. **"CD2AP is a biomarker for diabetic nephropathy progression"**
- **作者**：Gu C, et al.
- **摘要**：通过CD2AP抗体检测患者尿液中的CD2AP水平，发现其与糖尿病肾病严重程度相关，提出CD2AP作为疾病进展的潜在生物标志物。
以上研究均通过CD2AP抗体探索了该蛋白在疾病机制或临床诊断中的应用价值。
CD2-associated protein (CD2AP) is a multifunctional adaptor protein initially identified for its role in T-cell signaling through interaction with CD2. a surface receptor critical for immune synapse formation. Structurally, CD2AP contains three SH3 domains at its N-terminus and a coiled-coil domain at the C-terminus, enabling interactions with diverse signaling molecules, cytoskeletal components, and membrane proteins. Beyond immunology, CD2AP is essential in maintaining cellular structures, particularly in kidney podocytes, where it stabilizes the slit diaphragm by linking nephrin to the actin cytoskeleton. Mutations in CD2AP are associated with focal segmental glomerulosclerosis (FSGS) and chronic kidney disease.
CD2AP antibodies are vital tools for studying these mechanisms. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect CD2AP expression, localization, and protein interactions in tissues or cultured cells. In research, these antibodies help elucidate CD2AP's roles in endocytosis, receptor trafficking, and apoptosis regulation, particularly in neurodegenerative diseases like Alzheimer's, where CD2AP interacts with amyloid precursor protein. Commercially available CD2AP antibodies vary in specificity (polyclonal vs. monoclonal) and host species (rabbit, mouse), requiring validation for target applications. Emerging studies also explore CD2AP as a potential biomarker or therapeutic target in cancers and viral infections, underscoring its broad pathophysiological relevance.
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