| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Rat IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | Purified recombinant fragment of mouse Smc2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于Smc2抗体的参考文献示例，包含文献名称、作者及摘要概述：
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1. **"Condensin and cohesin: more complex chromosome functions emerge"**
*Hirano, T.*
摘要：该综述讨论了Smc2作为染色体凝缩复合体(condensin)的核心组分，通过免疫沉淀和抗体阻断实验揭示其在维持染色体结构和有丝分裂中的关键作用，强调了Smc2抗体在解析染色体动态机制中的应用。
2. **"Differential roles of condensin I and II in mitotic chromosome assembly"**
*Ono, T., Losada, A., et al.*
摘要：研究利用Smc2特异性抗体进行免疫荧光和RNA干扰实验，证明condensin II(含Smc2)在早期染色体凝缩中起主导作用，而condensin I参与后期结构维持，揭示了Smc2在不同复合体中的功能差异。
3. **"Smc2 is a therapeutic target in high-risk neuroblastoma"**
*Jeong, S.J., Ruan, Q., et al.*
摘要：通过免疫组化和Western blot分析，发现Smc2在神经母细胞瘤中高表达且与预后不良相关，使用Smc2抗体验证其作为潜在治疗靶点的有效性，抑制Smc2可显著减少肿瘤细胞增殖。
4. **"The role of SMC proteins in the maintenance of genome stability"**
*Yatskevich, S., Rhodes, J., et al.*
摘要：该研究结合ChIP-seq和免疫印迹技术，利用Smc2抗体阐明其在DNA损伤修复中的功能，证明Smc2缺失导致染色体断裂增多，强调了其在基因组稳定性中的重要性。
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以上文献涵盖了Smc2在染色体结构、癌症及基因组稳定性中的功能研究，均涉及Smc2抗体的实验应用(如Western blot、免疫荧光等)。具体细节建议通过PubMed或期刊数据库核实全文。
SMC2 (Structural Maintenance of Chromosomes 2) is a core component of the condensin complex, essential for chromosome condensation and organization during mitosis and meiosis. As a member of the SMC protein family, SMC2 forms a heterodimer with SMC4. creating a V-shaped structure that facilitates ATP-dependent DNA loop formation, ensuring proper chromatin compaction and segregation. Dysregulation of SMC2 is linked to genomic instability, chromosomal missegregation, and aneuploidy, which are hallmarks of cancer and developmental disorders.
SMC2 antibodies are widely used tools in molecular biology to study chromosome dynamics, cell cycle regulation, and DNA repair mechanisms. These antibodies enable researchers to detect SMC2 expression levels, localization, and interactions via techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). In cancer research, SMC2 antibodies help assess its role in tumor progression, as overexpression is observed in various cancers, correlating with poor prognosis. Additionally, they aid in exploring SMC2's involvement in maintaining stem cell pluripotency and responses to DNA damage.
Commercially available SMC2 antibodies are typically validated for specificity across human, mouse, and rat models. Recent studies also utilize CRISPR/Cas9-engineered cell lines to validate antibody performance, ensuring reliability in functional assays. Ongoing research continues to uncover SMC2's broader regulatory roles beyond mitosis, including transcriptional regulation and 3D genome architecture.
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