| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Rat IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | Purified recombinant fragment of mouse Ctcfl |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CtcfL(BORIS)抗体的3篇参考文献示例(内容为模拟生成，非真实文献)：
1. **文献名称**：BORIS: a novel CTCF homolog expressed in male germ cells
**作者**：Loukinov DI, et al.
**摘要**：本研究首次鉴定了BORIS(Brother of the Regulator of Imprinted Sites)作为CTCF的生殖细胞特异性旁系同源物。通过开发特异性抗体，证实其在睾丸组织中的表达定位，并揭示其可能参与生殖细胞表观遗传重编程。
2. **文献名称**：Antibody-based profiling of BORIS/CTCFL in human cancers
**作者**：Klenova EM, et al.
**摘要**：利用自主研发的BORIS单克隆抗体，本研究在多种癌症组织(如乳腺癌、肺癌)中检测到BORIS异常激活，与CTCF形成竞争性结合，可能导致基因组印记失调和癌基因异常表达。
3. **文献名称**：Structural characterization of CTCFL zinc finger domains using epitope-specific antibodies
**作者**：Ohlsson R, et al.
**摘要**：通过设计靶向Ctcfl锌指结构域的多克隆抗体，解析了其与CTCF在染色质结合位点的异同，发现BORIS优先结合富含GC的DNA区域，为理解其在表观遗传调控中的功能提供结构基础。
注：以上文献信息为基于领域知识生成的模拟内容，实际文献需通过PubMed/Google Scholar检索关键词"CTCFL antibody"或"BORIS antibody"获取。
**Background of CTCFL Antibody**
CTCFL (CTCF-like), also known as BORIS (Brother of the Regulator of Imprinted Sites), is a paralog of the chromatin organizer protein CTCF. While CTCF is ubiquitously expressed and regulates 3D genome architecture, transcriptional insulation, and imprinting, CTCFL expression is largely restricted to germ cells and certain cancers. CTCFL shares DNA-binding specificity with CTCF but exhibits distinct roles in epigenetic reprogramming, particularly during gametogenesis and oncogenesis.
Antibodies targeting CTCFL are critical tools for studying its unique functions in germ cell development, cancer biology, and epigenetic regulation. CTCFL is aberrantly activated in various cancers (e.g., melanoma, breast, and lung cancers), where it may antagonize CTCF-mediated tumor suppression, alter chromatin states, and promote oncogenic gene expression. Its role in testicular germ cell tumors and germline-specific imprinting further underscores its biological significance.
CTCFL antibodies enable detection of its expression patterns, chromatin localization (via ChIP-seq), and interactions with other proteins. They help distinguish CTCFL from CTCF in co-expression studies, given their overlapping DNA motifs but divergent biological contexts. Research using these antibodies has advanced understanding of CTCFL’s contribution to cancer plasticity, metastasis, and epigenetic instability, positioning it as a potential therapeutic target. However, challenges remain in elucidating its regulatory mechanisms and tissue-specific roles.
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