| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MAP3K1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4条关于 **MAP3K1抗体** 的参考文献(示例为虚构内容，实际文献需通过数据库检索)：
1. **文献名称**: *MAP3K1 regulates mitochondrial dynamics and apoptosis in cancer cells*
**作者**: Smith A, et al.
**摘要**: 研究利用MAP3K1特异性抗体，通过Western blot和免疫荧光技术，揭示MAP3K1通过调控线粒体分裂蛋白Drp1的磷酸化，促进肿瘤细胞凋亡的分子机制。
2. **文献名称**: *Functional analysis of MAP3K1 mutations in developmental disorders*
**作者**: Chen L, et al.
**摘要**: 通过免疫组化结合MAP3K1抗体，发现MAP3K1基因突变导致胚胎发育中ERK信号通路异常，与先天性肢体畸形相关。
3. **文献名称**: *MAP3K1-mediated signaling in immune response*
**作者**: Gupta R, et al.
**摘要**: 使用MAP3K1抗体进行流式细胞术分析，证明MAP3K1在T细胞活化中通过JNK通路调控炎症因子IL-2的分泌。
4. **文献名称**: *Antibody validation for MAP3K1 in heterogeneous tumor samples*
**作者**: Wang Y, et al.
**摘要**: 评估不同MAP3K1抗体的特异性及适用性，确认抗体在肿瘤组织中的可靠性，并应用于乳腺癌患者样本的预后分析。
(注：以上为示例模板，实际文献需通过PubMed、Google Scholar等平台检索关键词 "MAP3K1 antibody" + 研究领域。)
The MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1) antibody is a tool used to study the MAP3K1 protein, a serine/threonine kinase central to the MAPK signaling cascade. MAP3K1 regulates key cellular processes, including proliferation, differentiation, apoptosis, and stress responses, by activating downstream pathways such as JNK and ERK. Its gene, located on chromosome 5q11. encodes a protein with a kinase domain, a PB1 domain for protein interactions, and a caspase-3 cleavage site, reflecting its role in apoptosis.
Antibodies targeting MAP3K1 are widely employed in research to detect its expression, phosphorylation status, or localization in tissues and cell lines. These reagents help elucidate MAP3K1's involvement in diseases, particularly cancers (e.g., breast, ovarian) and developmental disorders. For instance, MAP3K1 mutations or dysregulation are linked to altered signaling in tumorigenesis and sexual differentiation anomalies. Studies using MAP3K1 antibodies have also explored its crosstalk with other pathways (e.g., Wnt, NF-κB) and responses to DNA damage or cytokines.
Validated antibodies are critical for techniques like Western blotting, immunohistochemistry, or immunofluorescence. Researchers prioritize antibodies with high specificity, often confirmed via knockout controls, to avoid cross-reactivity with homologous kinases (e.g., MAP3K2). Understanding MAP3K1's dual roles as a pro-survival or pro-apoptotic factor, depending on cellular context, remains a key focus, driving interest in therapeutic targeting and biomarker discovery.
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