| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NUMB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NUMB抗体的3篇参考文献(示例为虚构，仅用于格式演示)：
1. **文献名称**: "NUMB regulates endosomal degradation of Notch receptors to control neuronal development"
**作者**: Smith A, et al.
**摘要**: 研究利用NUMB抗体揭示其在神经元分化中通过调控Notch受体内体降解的机制，证明NUMB缺失导致Notch信号异常激活，影响神经干细胞命运。
2. **文献名称**: "NUMB suppresses breast cancer metastasis by inhibiting EMT via PTEN/AKT pathway"
**作者**: Chen L, et al.
**摘要**: 通过NUMB抗体检测发现，NUMB通过PTEN/AKT通路抑制上皮-间质转化(EMT)，低表达与乳腺癌转移和不良预后显著相关。
3. **文献名称**: "Differential roles of NUMB isoforms in stem cell maintenance and differentiation"
**作者**: Gupta R, et al.
**摘要**: 利用特异性NUMB抗体区分不同剪接异构体，发现NUMB长/短异构体在肠道干细胞自我更新与分化中具有相反作用，影响Wnt/β-catenin信号活性。
*注：以上文献信息为模拟生成，实际研究中请通过PubMed或Web of Science检索真实文献。*
NUMB Antibody Background:
The NUMB protein, encoded by the *NUMB* gene, is an evolutionarily conserved cell fate determinant critical for asymmetric cell division and cell signaling regulation. It functions as a key adaptor protein, primarily by inhibiting the Notch signaling pathway through interaction with the intracellular domain of Notch receptors, thereby influencing cell differentiation, proliferation, and survival. NUMB is essential in neurodevelopment, stem cell maintenance, and tissue homeostasis, with roles in processes such as neuronal differentiation, synaptic plasticity, and epithelial-mesenchymal transition (EMT).
NUMB exists as multiple isoforms generated by alternative splicing, resulting in protein variants with distinct molecular weights (e.g., 65–72 kDa or 75–115 kDa, depending on phosphorylation and isoforms). NUMB antibodies are widely used in research to detect these isoforms via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help elucidate NUMB's tissue-specific expression patterns, subcellular localization, and dysregulation in diseases.
Dysfunctional NUMB expression is linked to cancer (e.g., breast, lung, and colorectal cancers), where it often acts as a tumor suppressor, and neurodegenerative disorders like Alzheimer’s disease. NUMB antibodies also aid in studying mechanisms of drug resistance and regenerative medicine. When selecting NUMB antibodies, researchers must consider target epitopes, species reactivity (human, mouse, rat), and validation in specific experimental contexts to ensure specificity for intended isoforms.
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