| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NEDD4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NEDD4抗体的3篇参考文献示例(内容基于公开研究归纳，非真实文献)：
1. **"NEDD4 overexpression in colorectal cancer correlates with poor prognosis and promotes tumor growth"**
*作者：Zhang Y, et al.*
**摘要**：本研究通过Western blot和免疫组化(使用NEDD4特异性抗体)发现结直肠癌组织中NEDD4蛋白表达显著升高，且与患者生存率负相关。体外实验表明NEDD4通过泛素化降解PTEN促进肿瘤增殖。
2. **"NEDD4 regulates neuronal development via ubiquitination of synaptic proteins"**
*作者：Kawabe H, et al.*
**摘要**：利用NEDD4抗体进行免疫荧光和蛋白质分析，发现NEDD4在小鼠大脑皮层神经元中调控突触蛋白的泛素化修饰，敲除NEDD4导致突触形成异常，提示其在神经发育中的关键作用。
3. **"NEDD4-mediated ubiquitination of influenza A virus M2 protein enhances viral replication"**
*作者：Wang X, et al.*
**摘要**：研究通过免疫共沉淀(使用NEDD4抗体)证实流感病毒M2蛋白与NEDD4直接互作，NEDD4介导M2的泛素化修饰，增强病毒粒子释放，为抗病毒靶点开发提供依据。
*注：以上为模拟示例，实际文献需通过PubMed或Google Scholar检索关键词(如"NEDD4 antibody" + "Western blot"等)获取。*
The NEDD4 (Neural precursor cell Expressed, Developmentally Down-regulated 4) antibody is a critical tool for studying the NEDD4 protein, a member of the HECT-type E3 ubiquitin ligase family. NEDD4 plays a pivotal role in the ubiquitin-proteasome system, regulating substrate-specific protein degradation, intracellular trafficking, and signaling pathways. It contains distinct domains, including a C2 domain for membrane binding, multiple WW domains for substrate recognition, and a HECT domain responsible for catalyzing ubiquitin transfer. NEDD4 is involved in diverse cellular processes such as cell growth, apoptosis, and neural development, and its dysregulation has been linked to cancers, hypertension, and neurodegenerative diseases like Parkinson’s.
NEDD4 antibodies are widely used in research to detect and quantify NEDD4 expression in tissues or cells via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They also aid in studying post-translational modifications, protein-protein interactions, and functional mechanisms in disease models. Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple epitopes, enhancing sensitivity. Validated antibodies are essential to avoid cross-reactivity with homologous proteins (e.g., NEDD4L). Researchers rely on these antibodies to explore NEDD4’s role in degrading key targets (e.g., PTEN, EGFR) or modulating ion channels (e.g., ENaC), providing insights into therapeutic strategies targeting ubiquitination pathways. Proper validation via knockout controls or siRNA silencing is critical for experimental reliability.
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