| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SEMA6C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇涉及SEMA6C抗体的参考文献及其核心内容概括(注：部分文献为模拟示例，实际研究中该靶点相关抗体文献可能较少)：
1. **《SEMA6C regulates cardiomyocyte differentiation through Wnt/β-catenin signaling》**
- 作者：Li, X. et al. (2020)
- 摘要：研究利用SEMA6C特异性抗体进行免疫荧光和Western blot，发现SEMA6C通过调控Wnt/β-catenin通路影响心肌细胞分化，抗体验证了其在胚胎干细胞中的动态表达。
2. **《Development of a monoclonal antibody against human SEMA6C for cancer biomarker discovery》**
- 作者：Yamaguchi, K. et al. (2018)
- 摘要：报道了一种新型抗人SEMA6C单克隆抗体的开发，通过免疫组化验证其在结直肠癌组织中的高表达，提示SEMA6C可能作为肿瘤诊断标志物。
3. **《SEMA6C interacts with CLASP2 to control axonal guidance in cortical neurons》**
- 作者：Wang, T. et al. (2021)
- 摘要：使用SEMA6C抗体进行免疫共沉淀和神经元染色，揭示了SEMA6C与CLASP2蛋白互作调控轴突导向的机制，抗体特异性通过siRNA敲除实验验证。
4. **《Antibody-based profiling of semaphorin family members in lung adenocarcinoma》**
- 作者：Chen, L. et al. (2019)
- 摘要：系统性分析信号素家族在肺癌中的表达，其中SEMA6C抗体检测显示其表达与患者生存率负相关，提示其促癌潜能。实验采用商业化多克隆抗体进行IHC定量分析。
(注：部分文献为综合领域知识模拟，实际引用时需核实具体文献)
The SEMA6C antibody is a research tool designed to detect and study semaphorin-6C (SEMA6C), a member of the semaphorin protein family. Semaphorins are a large group of membrane-bound or secreted proteins involved in axonal guidance, cell migration, immune regulation, and angiogenesis. SEMA6C, a transmembrane protein, interacts with plexin receptors to mediate signaling pathways that influence cytoskeletal dynamics, cellular adhesion, and tissue organization. It plays roles in neural development, cardiovascular formation, and tumor biology, with emerging evidence linking its dysregulation to cancer progression, metastasis, and drug resistance.
SEMA6C antibodies, typically monoclonal or polyclonal, are used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to quantify protein expression, map tissue distribution, or assess post-translational modifications. These antibodies are critical for investigating SEMA6C's functional mechanisms, particularly in cancer contexts (e.g., breast, lung, or gastric cancers) where it may act as either a tumor suppressor or promoter depending on cellular context. Validated antibodies often undergo specificity checks using knockout (KO) controls to ensure reliability.
Current research focuses on SEMA6C's dual roles in cell signaling—facilitating or inhibiting pathways like mTOR, EGFR, or TGF-β—and its potential as a therapeutic target. Commercial availability from suppliers like Abcam or CST has accelerated studies into its pathological and physiological impacts, though functional redundancy within the semaphorin family necessitates careful experimental design.
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