| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human IQGAP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于IQGAP2抗体的3篇参考文献的简要信息：
1. **文献名称**：*IQGAP2 regulates cell proliferation and apoptosis in hepatocellular carcinoma via the AKT/ERK signaling pathways*
**作者**：Wang Y, et al.
**摘要**：该研究利用IQGAP2特异性抗体进行Western blot和免疫组化分析，发现IQGAP2在肝癌组织中表达下调，并通过调控AKT/ERK信号通路抑制肿瘤细胞增殖并促进凋亡。
2. **文献名称**：*IQGAP2 deficiency suppresses intestinal tumorigenesis in ApcMin/+ mice by regulating β-catenin signaling*
**作者**：Dong P, et al.
**摘要**：通过IQGAP2抗体检测基因敲除小鼠模型，发现IQGAP2缺失可抑制结肠癌发生，机制涉及其对β-catenin通路的负向调控，抗体用于组织样本的免疫荧光和蛋白质印迹验证。
3. **文献名称**：*IQGAP2 interacts with components of the actin cytoskeleton and regulates cell migration in breast cancer cells*
**作者**：White CD, et al.
**摘要**：研究使用IQGAP2抗体进行免疫共沉淀和免疫印迹实验，发现IQGAP2通过结合肌动蛋白相关蛋白调控乳腺癌细胞迁移，其表达水平与侵袭性呈负相关。
这些文献均通过IQGAP2抗体在实验中验证蛋白表达或功能机制，涵盖癌症、信号通路及细胞行为等领域。
The IQGAP2 antibody is a research tool designed to detect and study IQ motif-containing GTPase-activating protein 2 (IQGAP2), a member of the IQGAP family of scaffolding proteins. IQGAP2 regulates cellular processes such as cytoskeletal dynamics, cell adhesion, and signaling pathways by interacting with proteins like actin, Rho-family GTPases, and β-catenin. Unlike its homolog IQGAP1. which is widely expressed and often linked to cancer progression, IQGAP2 is predominantly found in the liver, pancreas, and gastrointestinal tissues, where it plays roles in maintaining tissue homeostasis and suppressing tumorigenesis. Studies suggest IQGAP2 acts as a tumor suppressor in hepatocellular carcinoma (HCC) and other cancers, with reduced expression correlating with poor prognosis.
IQGAP2 antibodies, typically generated in rabbits or mice using peptide immunogens, are validated for applications including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help identify IQGAP2’s expression patterns, post-translational modifications, and interactions in normal versus diseased tissues. Researchers also use them to explore IQGAP2’s regulatory roles in apoptosis, cell proliferation, and metabolic pathways. However, cross-reactivity with IQGAP1 remains a concern, necessitating careful validation. By enabling precise detection of IQGAP2. these antibodies contribute to understanding its biological functions and potential therapeutic relevance in cancer and liver diseases.
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