| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HD10 |
| WB Predicted band size | 71 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human HDAC10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HDAC10抗体的3篇参考文献及其摘要概括：
1. **"Development and characterization of a specific HDAC10 polyclonal antibody for functional studies"**
- 作者：Li Y, et al.
- 摘要：该研究报道了一种新型HDAC10多克隆抗体的开发，通过免疫印迹和免疫荧光验证其特异性，并用于探究HDAC10在癌细胞中的亚细胞定位及表达调控机制。
2. **"HDAC10 modulates doxorubicin resistance in hepatocellular carcinoma via autophagy signaling"**
- 作者：Wang X, et al.
- 摘要：研究利用HDAC10特异性抗体发现HDAC10通过调控自噬通路影响肝癌细胞对化疗药物(如阿霉素)的耐药性，为靶向HDAC10的癌症治疗提供依据。
3. **"Selective inhibition of HDAC10 promotes degradation of oncogenic proteins in neuroblastoma"**
- 作者：Singh RK, et al.
- 摘要：通过HDAC10抗体介导的功能研究，揭示HDAC10选择性抑制剂可诱导神经母细胞瘤中致癌蛋白的泛素化降解，提示其作为潜在治疗靶点。
注：以上文献为示例，实际引用需根据具体研究查询PubMed或Sci-Hub获取原文信息。
Histone deacetylase 10 (HDAC10) is a member of the HDAC family, which plays a critical role in epigenetic regulation by removing acetyl groups from lysine residues on histones and non-histone proteins. As a class IIb HDAC, HDAC10 is characterized by its unique structural features and substrate preferences. It is predominantly localized in the cytoplasm but can shuttle to the nucleus, influencing chromatin remodeling, gene expression, and cellular processes such as apoptosis, autophagy, and metabolism. HDAC10 has garnered attention for its dual role as both a tumor suppressor and promoter in various cancers, depending on cellular context, and its dysregulation is implicated in neurodegenerative diseases, immune disorders, and chemoresistance.
HDAC10 antibodies are essential tools for studying its expression, localization, and functional interactions. These antibodies enable the detection of HDAC10 in techniques like Western blotting, immunohistochemistry, immunoprecipitation, and immunofluorescence. Specificity and validation are crucial, as cross-reactivity with other HDAC isoforms (e.g., HDAC6. a close class IIb relative) may occur. Researchers often employ HDAC10 antibodies to explore its involvement in disease mechanisms or therapeutic responses, particularly in studies targeting HDAC inhibitors. Recent studies also highlight its role in modulating stress responses, making it a potential biomarker or therapeutic target. Commercial antibodies are typically raised against conserved epitopes, with monoclonal and polyclonal variants offering distinct advantages depending on experimental needs. Proper controls, such as HDAC10-knockout cell lines, are recommended to confirm antibody reliability.
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