| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RCC; SCAR26 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human XRCC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与XRCC1抗体相关的代表性文献，按发表时间排序：
1. **"XRCC1 protein: Form and function"**
作者：K.W. Caldecott (2019)
摘要：系统综述XRCC1蛋白在DNA单链断裂修复中的分子机制，重点讨论了其抗体在检测细胞核内修复复合体定位的应用，并比较了不同商业抗体的特异性验证方法。
2. **"Validation of XRCC1 antibodies for immunohistochemical analysis of breast cancer tissues"**
作者：M. García-Suárez等 (2017)
摘要：通过Western blot和免疫组化验证两种商用XRCC1抗体(EPR219和12B)的特异性，发现EPR219抗体在乳腺癌组织中检测XRCC1蛋白表达的可靠性更高，且与患者预后相关。
3. **"XRCC1 genetic polymorphism influences antibody-based detection of protein expression patterns"**
作者：T. Yan等 (2015)
摘要：研究发现XRCC1基因Arg399Gln多态性会影响抗体识别效率，携带Gln等位基因的样本中部分抗体出现假阴性现象，提示基因型需纳入实验设计考量。
4. **"Development of monoclonal antibodies targeting the N-terminal domain of XRCC1"** (经典文献)
作者：S. Nakamura等 (2000)
摘要：首次报道制备针对XRCC1蛋白N端结构域的小鼠单克隆抗体，证实其可用于免疫沉淀和损伤修复复合物的共定位分析，奠定后续研究基础。
注：以上为模拟文献，实际引用请通过PubMed/Web of Science检索最新文献。建议关注《DNA Repair》《Nucleic Acids Research》等期刊近年发表的抗体验证相关研究。
The XRCC1 (X-ray repair cross-complementing protein 1) antibody is a crucial tool for studying DNA repair mechanisms. XRCC1 is a scaffold protein essential for base excision repair (BER) and single-strand break repair (SSBR), interacting with key enzymes like PARP1. DNA ligase III, and DNA polymerase β to maintain genomic stability. It lacks enzymatic activity but coordinates repair processes by facilitating protein-protein interactions and recruiting repair components to damaged sites.
XRCC1 antibodies are widely used in research to detect and quantify XRCC1 expression in cells and tissues, employing techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help elucidate XRCC1's role in cellular responses to DNA damage induced by radiation, oxidative stress, or chemotherapeutic agents. Dysregulation of XRCC1 is linked to genomic instability, cancer susceptibility, and chemotherapy resistance, making it a biomarker of interest in oncology.
Studies using XRCC1 antibodies have revealed its variable expression across cancers, with implications for patient prognosis and treatment response. Additionally, XRCC1 polymorphisms, associated with altered DNA repair capacity and cancer risk, are investigated using antibody-based assays. However, antibody specificity and variability across commercial sources require careful validation to ensure experimental reproducibility. Overall, XRCC1 antibodies are vital for advancing understanding of DNA repair pathways and their therapeutic targeting.
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