| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ABH5; OFOXD; OFOXD1 |
| WB Predicted band size | 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ALKBH5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇涉及ALKBH5抗体的代表性文献摘要：
---
1. **文献名称**：*ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility*
**作者**：Zheng G. et al. (2013)
**摘要**：该研究首次证实ALKBH5作为哺乳动物m6A RNA去甲基酶的功能，通过特异性抗体检测其在睾丸组织中的高表达，并揭示其缺失导致精子形成缺陷及雄性不育。
---
2. **文献名称**：*Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m6A-demethylation*
**作者**：Zhang C. et al. (2016)
**摘要**：研究利用ALKBH5抗体进行免疫印迹和免疫荧光，证明低氧通过HIF-ALKBH5通路降低m6A水平，促进乳腺癌干细胞特性，揭示了ALKBH5在肿瘤微环境中的调控机制。
---
3. **文献名称**：*Dynamic m6A mRNA methylation directs T cell homeostasis*
**作者**：Li H. et al. (2017)
**摘要**：通过ALKBH5抗体进行免疫共沉淀实验，发现ALKBH5介导的m6A修饰动态调控T细胞活化基因的翻译效率，影响T细胞稳态及自身免疫疾病发展。
---
如需具体实验抗体信息(如品牌、货号)，建议进一步查阅抗体供应商(如Abcam、CST)的产品引用文献。
The ALKBH5 antibody is a crucial tool for studying the ALKBH5 protein, a member of the AlkB homolog (ALKBH) family of dioxygenases. ALKBH5 specifically catalyzes the demethylation of N6-methyladenosine (m6A), a dynamic and reversible RNA modification pivotal in post-transcriptional gene regulation. Discovered in 2011. ALKBH5 is predominantly localized in the nucleus and plays a key role in RNA metabolism, influencing processes such as mRNA splicing, stability, translation, and nuclear export. Its function in m6A erasure links it to diverse biological pathways, including stem cell differentiation, tumorigenesis, immune responses, and neurological disorders. Dysregulation of ALKBH5 has been implicated in cancers (e.g., glioblastoma, breast cancer), infertility, and metabolic diseases, highlighting its therapeutic potential.
ALKBH5 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP) to detect protein expression, localization, and interactions. High-quality antibodies are validated for specificity (e.g., via knockout controls) and cross-reactivity (e.g., human, mouse). Researchers rely on these antibodies to explore ALKBH5's role in m6A-mediated epigenetic regulation and its association with disease mechanisms. For instance, ALKBH5 inhibitors are being investigated as potential anticancer agents. As m6A research expands, ALKBH5 antibodies remain essential for unraveling RNA modification networks and their impact on cellular physiology and pathology.
×
