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产品描述：人组蛋白H3.1(HIST1H3A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HIST1H3A含量。HIST1H3A是一种组蛋白基因。它在染色质结构和基因表达调控中起关键作用。研究中，其与细胞增殖、分化等密切相关。异常表达可能影响细胞正常生理功能，引发疾病，目前对其作用机制深入研究有助于探索相关疾病治疗靶点。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，支持表观遗传学、疾病分子机制、药物筛选等基础研究，例如探究H3.1在肿瘤模型中的动态修饰、细胞周期相关蛋白互作或特定病理状态下组蛋白表达谱变化。其兼容多种生物样本的特性，为体外实验与体内研究的数据关联分析提供便利，适用于分子生物学、细胞生物学等领域的定量研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：H3 histone family member E pseudogene ELISA Kit; H3 histone family; member A ELISA Kit; H3/A ELISA Kit; H31_HUMAN ELISA Kit; H3F3 ELISA Kit; H3FA ELISA Kit; Hist1h3a ELISA Kit; HIST1H3B ELISA Kit; HIST1H3C ELISA Kit; HIST1H3D ELISA Kit; HIST1H3E ELISA Kit; HIST1H3F ELISA Kit; HIST1H3G ELISA Kit; HIST1H3H ELISA Kit; HIST1H3I ELISA Kit; HIST1H3J ELISA Kit; HIST3H3 ELISA Kit; histone 1; H3a ELISA Kit; Histone cluster 1; H3a ELISA Kit; Histone H3 3 pseudogene ELISA Kit; Histone H3.1 ELISA Kit; Histone H3/a ELISA Kit; Histone H3/b ELISA Kit; Histone H3/c ELISA Kit; Histone H3/d ELISA Kit; Histone H3/f ELISA Kit; Histone H3/h ELISA Kit; Histone H3/i ELISA Kit; Histone H3/j ELISA Kit; Histone H3/k ELISA Kit; Histone H3/l ELISA Kit
缩写：HIST1H3A
Uniprot No.：P68431
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HIST1H3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-93 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 80-95 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-98 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of human HIST1H3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-105 | ||||
| EDTA plasma (n=4) | 92 | 87-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 15002.1572.1342.1462.004 7501.6231.5621.5931.451 3751.1581.0821.1200.978 187.50.7460.6970.7220.580 940.4710.4510.4610.319 470.3480.3210.3350.193 23.50.2530.2490.2510.109 00.1480.1360.142 | ||||||
货期：3-5 working days
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