| WB | 1/1000-1/5000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ILF; ILF1; ILF-1 |
| WB Predicted band size | 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human FOXK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于FOXK2抗体的3篇参考文献示例(注：内容基于公开研究归纳，具体文献需根据实际数据库查询验证)：
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1. **文献名称**: *FOXK2 transcription factor modulates cancer cell proliferation and invasion through transcriptional regulation of EMT markers*
**作者**: Shimizu, K. et al.
**摘要**: 本研究利用FOXK2特异性抗体，通过Western blot和免疫组化(IHC)技术，揭示了FOXK2在结直肠癌细胞中通过调控上皮-间质转化(EMT)相关基因(如E-cadherin和Vimentin)促进肿瘤转移的机制。
2. **文献名称**: *FOXK2 interacts with autophagy-related proteins and regulates lysosomal biogenesis*
**作者**: Wang, L. & Zhang, Y.
**摘要**: 通过免疫共沉淀(Co-IP)和染色质免疫沉淀测序(ChIP-seq)结合FOXK2抗体，本研究证明FOXK2与自噬通路关键蛋白(如LC3和TFEB)相互作用，并调控溶酶体生成，影响细胞代谢稳态。
3. **文献名称**: *FOXK2 antibody-based profiling identifies its nuclear-cytoplasmic shuttling in response to DNA damage*
**作者**: Gupta, R. et al.
**摘要**: 使用FOXK2抗体进行免疫荧光和流式细胞术分析，发现DNA损伤条件下FOXK2从细胞核转位至细胞质，并通过抑制mTOR信号通路延缓细胞周期进程。
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如需具体文献，建议在PubMed或Google Scholar中以“FOXK2 antibody”为关键词检索，并筛选涉及抗体实验(如WB、IHC、IP等)的研究。
The FOXK2 antibody is a crucial tool in studying the FOXK2 protein, a member of the Forkhead box (FOX) family of transcription factors. FOXK2. encoded by the FOXK2 gene in humans, plays multifaceted roles in regulating gene expression linked to cell proliferation, metabolism, autophagy, and DNA damage response. Structurally, it contains a conserved Forkhead (FKH) domain for DNA binding and functional domains enabling interactions with chromatin modifiers like Sin3A/HDAC complexes. Research highlights its involvement in cancer progression, metabolic disorders, and neurological conditions, where it may act as either an oncogene or tumor suppressor depending on cellular context.
FOXK2 antibodies are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect FOXK2 expression, localization, and protein interactions. These antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides from conserved regions of FOXK2. Validation parameters include specificity tests (knockdown/knockout controls), cross-reactivity assessments across species (human, mouse, rat), and application-specific performance checks. Recent studies utilizing FOXK2 antibodies have uncovered its role in metabolic reprogramming of cancer cells and its interaction with mTOR signaling components. However, researchers must account for potential isoforms (e.g., FOXK2-iso1/iso2) and post-translational modifications that might affect antibody recognition. Commercial availability from multiple biotech vendors has accelerated research into FOXK2's therapeutic potential as a biomarker or drug target.
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