首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠高密度脂蛋白(HDL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的HDL含量。高密度脂蛋白（HDL）是一种重要的血清脂蛋白，其代谢与重塑过程受到多种分子调节。研究显示，HDL在胆固醇代谢中发挥关键作用，通过抑制胆固醇在血管壁的蓄积，减轻动脉粥样硬化。目前，针对HDL代谢与重塑的研究正不断深入，旨在开发相关药物以更全面评价HDL的抗动脉粥样硬化作用。试剂盒检测范围为78 ng/mL-5000 ng/mL，适用于心血管疾病模型研究、脂代谢机制探索、药物筛选或营养干预评估等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：HDL
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：78 ng/mL-5000 ng/mL
灵敏度：19.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:400 | Average % | 103 | ||||
| Range % | 100-107 | |||||
| 1:800 | Average % | 89 | ||||
| Range % | 84-96 | |||||
| 1:1600 | Average % | 96 | ||||
| Range % | 94-97 | |||||
回收率：
| The recovery of rat HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-99 | ||||
| EDTA plasma (n=4) | 103 | 99-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 50002.5112.6202.5662.444 25001.8631.8701.8671.745 12501.3441.2361.2901.168 6250.7560.7870.7720.650 312.50.5210.5600.5410.419 1560.3580.3560.3570.235 780.2260.2300.2280.106 00.1180.1250.122 | ||||||
货期：3-5 working days
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