| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AOS4; AER61; EOGT1; C3orf64 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human EOGT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EOGT抗体的3篇代表性文献示例(内容为模拟概括，仅供参考)：
1. **"EOGT regulates Notch signaling by modulating O-linked glycosylation in Drosophila"**
- 作者：Ogawa M, et al.
- 摘要：研究通过果蝇模型，利用EOGT抗体检测其表达分布，揭示EOGT通过O-GlcNAc糖基化修饰调控Notch信号通路的活性，影响发育过程。
2. **"EOGT deficiency disrupts epidermal barrier formation in humans"**
- 作者：Takeuchi H, et al.
- 摘要：通过免疫荧光和Western blot技术(使用EOGT抗体)，发现人类皮肤中EOGT缺失导致表皮屏障功能异常，可能与先天性皮肤疾病相关。
3. **"Mutations in EOGT link glycosylation defects to Adams-Oliver syndrome"**
- 作者：Sawkin PJ, et al.
- 摘要：研究利用EOGT抗体分析患者细胞，发现EOGT基因突变导致蛋白糖基化异常，与Adams-Oliver综合征的血管和肢体发育缺陷密切相关。
(注：以上文献信息为示例，实际引用需以真实文献为准。建议通过PubMed或Google Scholar以“EOGT antibody”或“EOGT function”为关键词检索最新研究。)
EOGT (Epidermal Growth Factor-like domain-specific O-linked N-acetylglucosamine Transferase) is a glycosyltransferase that catalyzes the addition of O-linked N-acetylglucosamine (O-GlcNAc) to specific serine or threonine residues within epidermal growth factor-like (EGF-like) domains of proteins. This post-translational modification occurs in the endoplasmic reticulum and is distinct from cytoplasmic O-GlcNAcylation. EOGT plays a critical role in regulating cell-surface receptors, particularly those involved in the Notch signaling pathway, which influences cellular differentiation, proliferation, and tissue development. Mutations in the EOGT gene have been linked to Adams-Oliver syndrome, a rare genetic disorder characterized by congenital scalp defects and vascular abnormalities, highlighting its importance in embryonic development.
EOGT antibodies are essential tools for studying the expression, localization, and function of EOGT in various biological contexts. They enable researchers to investigate its interaction with substrates, such as NOTCH receptors, and its role in diseases. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to visualize EOGT in tissues or cultured cells. Validation of antibody specificity is crucial, as cross-reactivity with other glycosyltransferases or O-GlcNAc-related proteins may occur. Recent studies also explore EOGT's potential involvement in cancer and metabolic disorders, expanding its relevance beyond developmental biology. Understanding EOGT-mediated O-GlcNAcylation provides insights into glycosylation-dependent cellular communication and disease mechanisms.
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