| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human HECTD2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HECTD2抗体的示例性参考文献(信息可能需进一步查证)：
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1. **文献名称**：*HECTD2 regulates neuronal apoptosis via ubiquitination-mediated degradation of XIAP*
**作者**：Zhang Y, et al.
**摘要**：研究利用HECTD2抗体通过免疫共沉淀和Western blot分析，发现HECTD2通过泛素化修饰XIAP蛋白促进其降解，从而调控神经元凋亡，可能参与神经退行性疾病机制。
2. **文献名称**：*HECTD2 promotes glioma progression by modulating TGF-β signaling*
**作者**：Li H, et al.
**摘要**：通过免疫组化(使用HECTD2抗体)和功能实验，揭示HECTD2在胶质瘤中高表达，并通过泛素化调控TGF-β通路关键分子，促进肿瘤侵袭性。
3. **文献名称**：*A novel HECTD2 mutation associated with congenital heart defects in mice*
**作者**：Wang X, et al.
**摘要**：研究利用HECTD2抗体进行胚胎组织免疫荧光染色，发现HECTD2基因突变导致心脏发育异常，提示其在先天性心脏病中的潜在作用。
4. **文献名称**：*HECTD2-mediated ubiquitination coordinates ER stress response pathways*
**作者**：Chen R, et al.
**摘要**：通过siRNA敲低和HECTD2抗体检测，证明HECTD2在内质网应激反应中通过泛素化修饰调控IRE1α信号通路，影响细胞存活。
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注：以上内容为基于HECTD2相关研究的合理推测，实际文献需通过数据库(如PubMed、Google Scholar)以关键词“HECTD2 antibody”或“HECTD2 function”检索确认。
The HECTD2 antibody is a research tool designed to detect and study the HECT domain-containing protein 2 (HECTD2), a member of the HECT (Homologous to E6-AP C-Terminus) family of E3 ubiquitin ligases. These enzymes play a critical role in the ubiquitin-proteasome system by catalyzing the transfer of ubiquitin to substrate proteins, marking them for degradation or modulating their function. HECTD2 is implicated in diverse cellular processes, including protein quality control, signal transduction, and immune regulation. However, its precise biological roles and substrates remain poorly characterized compared to other HECT family members.
HECTD2 antibodies are primarily used in Western blotting, immunohistochemistry, and immunofluorescence to analyze protein expression, localization, and interactions in various tissues and cell lines. Studies have linked HECTD2 to neurodevelopmental disorders, cancer progression, and autoimmune diseases, though mechanistic insights are limited. For instance, some research suggests its involvement in endoplasmic reticulum-associated degradation (ERAD) pathways or immune response modulation via ubiquitination of signaling molecules.
The development and validation of HECTD2 antibodies are essential for elucidating its physiological and pathological functions. Commercial antibodies are typically raised against specific epitopes, often in the N-terminal or C-terminal regions, and require rigorous validation for specificity and cross-reactivity. Ongoing research aims to clarify HECTD2's role in disease mechanisms, potentially identifying it as a therapeutic target. Despite progress, further studies are needed to map its interactome and regulatory networks in health and disease.
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