| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EULIR |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human HECTD1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HECTD1抗体的3篇参考文献及其摘要概括：
1. **标题**：*HECTD1调控自噬-溶酶体通路在神经管缺陷中的作用*
**作者**：Smith J, et al.
**摘要**：研究通过HECTD1抗体进行免疫沉淀和Western blot分析，发现HECTD1通过泛素化修饰调控自噬关键蛋白，其功能缺失导致小鼠胚胎神经管闭合异常，提示其在神经管缺陷中的机制。
2. **标题**：*HECTD1介导的TRAF3泛素化促进乳腺癌转移*
**作者**：Chen L, et al.
**摘要**：利用HECTD1抗体检测蛋白表达水平，发现HECTD1通过K63泛素化增强TRAF3稳定性，激活NF-κB通路，促进乳腺癌细胞侵袭，为靶向治疗提供新方向。
3. **标题**：*HECTD1与新冠病毒N蛋白互作抑制宿主抗病毒免疫*
**作者**：Wang Q, et al.
**摘要**：研究采用HECTD1抗体进行免疫共沉淀实验，揭示新冠病毒N蛋白劫持HECTD1泛素连接酶活性，降解MAVS蛋白，逃逸宿主天然免疫应答的分子机制。
(注：以上文献为虚拟示例，实际引用需检索PubMed等数据库获取真实文献。)
The HECTD1 antibody targets the HECT domain E3 ubiquitin protein ligase 1 (HECTD1), a member of the HECT family of E3 ubiquitin ligases involved in the ubiquitin-proteasome system. HECTD1 plays critical roles in regulating protein degradation, intracellular trafficking, and cellular signaling pathways. It is implicated in diverse biological processes, including endoplasmic reticulum-associated degradation (ERAD), neural development, and immune responses. Structurally, HECTD1 contains an N-terminal domain for substrate recognition and a C-terminal HECT domain responsible for ubiquitin transfer.
Research using HECTD1 antibodies has highlighted its involvement in diseases such as cancer, neurodevelopmental disorders, and congenital defects. For instance, HECTD1 dysregulation has been linked to tumor progression in breast cancer and glioblastoma, where it modulates oncogenic pathways or tumor suppressor proteins. In neurodevelopment, HECTD1 mutations are associated with neural tube defects, and its role in regulating neural crest cell migration underscores its importance in embryogenesis.
HECTD1 antibodies are widely utilized in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to study protein expression, localization, and interaction partners. Commercial antibodies vary in specificity, often validated across species (human, mouse, rat) for research or diagnostic applications. Ongoing studies explore HECTD1's potential as a therapeutic target or biomarker, emphasizing its multifaceted role in cellular homeostasis and disease mechanisms.
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