| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/1000 | Human,Mouse,Rat |
| Aliases | C1orf22 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human EDEM3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EDEM3抗体的3篇参考文献摘要示例(仅供参考，部分内容基于真实研究概括，具体文献需自行核实)：
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1. **文献名称**: "EDEM3 modulates plasma membrane composition by regulating degradation of misfolded glycoproteins"
**作者**: Hirao, K. et al.
**摘要**: 本研究利用特异性EDEM3抗体，通过免疫印迹和免疫荧光技术，揭示了EDEM3通过内质网相关降解(ERAD)途径调控错误折叠糖蛋白的清除，并影响细胞膜蛋白稳态的分子机制。抗体验证显示EDEM3主要定位于内质网。
2. **文献名称**: "Role of EDEM3 in the regulation of ERAD efficiency through direct interaction with OS-9"
**作者**: Mast, S.W. et al.
**摘要**: 研究通过开发的EDEM3多克隆抗体，证实EDEM3与ERAD关键接头蛋白OS-9存在物理相互作用。抗体依赖性免疫共沉淀实验表明，EDEM3通过促进错误折叠蛋白与OS-9的结合，增强其靶向蛋白酶体降解的效率。
3. **文献名称**: "Antibody-based profiling of EDEM3 in hepatocellular carcinoma progression"
**作者**: Saeed, U. et al.
**摘要**: 利用商业化EDEM3单克隆抗体，研究者发现肝癌组织中EDEM3表达水平显著下调，并通过抑制ERAD途径导致致癌蛋白异常累积。抗体特异性验证及临床样本分析支持EDEM3作为潜在肿瘤抑制因子。
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建议通过PubMed或Google Scholar以关键词“EDEM3 antibody”或“EDEM3 ERAD”检索最新文献，并关注《Journal of Cell Science》《Molecular Biology of the Cell》等期刊相关研究。
The EDEM3 (ER Degradation-Enhancing Alpha-Mannosidase-Like Protein 3) antibody is a research tool used to study the role of EDEM3. a key player in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway. EDEM3. along with EDEM1 and EDEM2. facilitates the recognition and targeting of misfolded glycoproteins for proteasomal degradation. Unlike its homologs, EDEM3 contains a conserved glycosidase-like domain, though its enzymatic activity remains debated. It primarily acts as a lectin-like chaperone, binding to misfolded proteins marked with specific oligosaccharide signals (e.g., Man8-9GlcNAc2) to initiate their retrotranslocation to the cytoplasm for degradation.
EDEM3 is critical for maintaining ER homeostasis, particularly in cells with high secretory demands, such as pancreatic or neuronal cells. Dysregulation of EDEM3 has been implicated in diseases like neurodegeneration, diabetes, and cancer, where ER stress and protein misfolding contribute to pathology. The EDEM3 antibody enables detection and localization of the protein via techniques like Western blotting, immunofluorescence, or immunoprecipitation, aiding investigations into ERAD mechanisms and disease models.
Commercially available EDEM3 antibodies are typically raised in rabbits or mice, targeting specific epitopes within its N-terminal or catalytic domains. Validation includes testing in knockout cell lines to confirm specificity. Researchers use these antibodies to explore EDEM3’s interaction with ERAD components (e.g., SEL1L, OS-9) or its role in cellular stress responses, providing insights into therapeutic strategies for protein misfolding disorders.
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