首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠骨形态形成蛋白拮抗蛋白酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GREM1含量。GREM1作为靶点，在机体胚胎发育、组织修复等过程中发挥重要作用。研究发现它能通过调控BMP信号通路等影响细胞的增殖、分化和凋亡，与多种疾病如肿瘤、骨疾病等发生发展相关，对其深入研究有助于开发新的治疗策略和药物。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于体外培养细胞模型、动物实验样本中BMP拮抗蛋白的定量分析，可支持骨骼生物学研究、信号通路调控机制探索以及相关疾病的分子机制解析，为药物干预效果评估或病理模型构建提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Grem1 ELISA Kit; Cktsf1b1 ELISA Kit; Drm ELISA Kit; Gremlin-1 ELISA Kit; Cysteine knot superfamily 1 ELISA Kit; BMP antagonist 1 ELISA Kit; Down-regulated in Mos-transformed cells protein ELISA Kit
缩写：GREM1
Uniprot No.：O35793
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat gremlin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 101-109 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 94-98 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 83-95 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
回收率：
| The recovery of rat gremlin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 99-104 | |||||
| EDTA plasma (n=4) | 90 | 85-96 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected1002.2102.2222.2162.094501.7981.8441.8211.699251.4321.5031.4681.34612.51.0531.0621.0580.9366.250.7510.7220.7370.6153.120.3910.4110.4010.2791.560.2580.2660.2620.14000.1210.1230.122 | |||||||
货期：3-5 working days
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