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产品描述：人Gremlin-1蛋白(GREM1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的GREM1含量。GREM1是一个重要靶点。它在多种生理和病理过程中发挥作用。研究机制方面，其参与调控细胞增殖、分化等。在疾病研究里，与肿瘤、纤维化等疾病的发生发展相关，为开发针对GREM1的治疗策略，以改善疾病治疗效果提供了方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于疾病机制研究、体外药物干预效果评估或病理模型中GREM1表达动态监测等场景，例如纤维化疾病模型中的蛋白表达分析、肿瘤微环境相关研究以及靶向治疗药物的体外活性测试本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BMP antagonist 1 ELISA Kit; Cell proliferation inducing gene 2 protein ELISA Kit; Cell proliferation-inducing gene 2 protein ELISA Kit; CKTSF1B1 ELISA Kit; colorectal adenoma and carcinoma 1 ELISA Kit; Cysteine knot superfamily 1 ELISA Kit; Cysteine knot superfamily 1; BMP antagonist 1 ELISA Kit; Cysteine knot superfamily BMP antagonist 1 ELISA Kit; DAN domain family member 2 ELISA Kit; DAND2 ELISA Kit; Down regulated in Mos-transformed cells protein ELISA Kit; Down-regulated in Mos-transformed cells protein ELISA Kit; DRM ELISA Kit; grem1 ELISA Kit; GREM1_HUMAN ELISA Kit; Gremlin 1 homolog; cysteine knot superfamily ELISA Kit; Gremlin 1 like protein ELISA Kit; Gremlin 1; cysteine knot superfamily; homolog ELISA Kit; GREMLIN ELISA Kit; Gremlin-1 ELISA Kit; Gremlin1 ELISA Kit; IHG-2 ELISA Kit; IHG2 ELISA Kit; Increased in high glucose 2 ELISA Kit; Increased in high glucose protein 2 ELISA Kit; PIG2 ELISA Kit; Proliferation inducing gene 2 ELISA Kit; proliferation inducing gene 2 protein ELISA Kit
缩写：GREM1
Uniprot No.：O60565
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GREM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-92 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-99 | |||||
回收率：
| The recovery of human GREM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 84-96 | ||||
| EDTA plasma (n=4) | 87 | 82-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4872.5862.5372.40452.2852.2962.2912.1582.51.8031.9231.8631.7301.251.3711.4321.4021.2690.6250.8690.8890.8790.7460.3120.5810.5870.5840.4510.1560.1760.1840.1800.04700.1320.1340.133 | ||||||
货期：3-5 working days
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