| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SCN2; GFI-1; GFI1A; ZNF163 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GFI1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GFI1抗体的示例参考文献(内容为模拟合成，仅供参考)：
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1. **文献名称**：*GFI1 Antibody Validation for Hematopoietic Stem Cell Analysis*
**作者**：Smith A, et al.
**摘要**：本研究开发并验证了一种高特异性GFI1单克隆抗体，通过Western blot和免疫荧光技术证实其在造血干细胞中的表达定位。抗体在小鼠模型中成功用于追踪GFI1在髓系分化中的动态变化，为造血系统研究提供工具支持。
2. **文献名称**：*Role of GFI1 in T-cell Lymphoma: Insights from Antibody-based Detection*
**作者**：Johnson RL, et al.
**摘要**：通过商业化GFI1多克隆抗体，研究团队分析了T细胞淋巴瘤患者样本中GFI1蛋白的异常表达，发现其与肿瘤侵袭性相关，并揭示了GFI1通过调控下游靶基因促进淋巴瘤发展的分子机制。
3. **文献名称**：*GFI1 as a Biomarker in Acute Myeloid Leukemia: Comparative Antibody Study*
**作者**：Chen H, et al.
**摘要**：对比三种GFI1抗体的灵敏度和特异性，确定某克隆号抗体在流式细胞术中效果最佳。研究证实GFI1低表达与AML患者预后不良相关，为临床检测提供了优化方案。
4. **文献名称**：*Epigenetic Regulation by GFI1 in Neural Crest Cells: Immunohistochemical Approach*
**作者**：Martinez D, et al.
**摘要**：利用GFI1抗体进行组织染色，揭示了其在神经嵴细胞分化中的表观遗传调控作用，提示GFI1通过抑制特定基因维持细胞未分化状态，为发育生物学研究提供新视角。
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**注**：以上文献为示例，实际引用需查询PubMed、Web of Science等数据库获取真实研究。如需具体文献，可补充关键词或研究场景进一步筛选。
The GFI1 (Growth Factor Independent 1) antibody is a crucial tool for studying the GFI1 protein, a zinc finger transcription repressor involved in hematopoiesis, immune regulation, and neurodevelopment. GFI1 plays a dual role in cell differentiation and oncogenesis by recruiting chromatin modifiers (e.g., histone deacetylases) to suppress target genes. It is essential for neutrophil, B-cell, and erythroid development, while its dysregulation links to leukemia, lymphomas, and neurological disorders.
GFI1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and flow cytometry to detect GFI1 expression patterns in tissues or cell lines. Researchers employ these antibodies to explore GFI1's role in stem cell maintenance, cancer progression, and immune responses. Commercial antibodies are typically raised against specific epitopes (e.g., human GFI1's N-terminal region) and validated in knockout models to ensure specificity.
Challenges include cross-reactivity with homologs (e.g., GFI1B) or non-specific binding due to protein isoforms. Recent studies using GFI1 antibodies have uncovered its tumor-suppressive functions in T-cell malignancies and its interplay with oncogenic pathways like MYC. Proper controls, such as GFI1-deficient cells, are critical for interpreting experimental results. Overall, GFI1 antibodies remain indispensable for dissecting its complex roles in health and disease.
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