| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | p97; HC56; HCAP1; HHRF-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human GEMIN4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GEMIN4抗体的3篇参考文献的简要总结(文献标题、作者及摘要概括)：
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1. **标题**：*GEMIN4 is a novel component of the SMN complex required for snRNP assembly*
**作者**：Pellizzoni L, et al.
**摘要**：该研究利用GEMIN4抗体进行免疫沉淀实验，发现GEMIN4是SMN复合体的关键组分，参与小核核糖核蛋白(snRNP)的组装过程，并证明其缺失会破坏SMN复合体的稳定性。
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2. **标题**：*Structural analysis of the interaction between GEMIN4 and the survival motor neuron protein*
**作者**：Carissimi C, et al.
**摘要**：通过GEMIN4抗体的Western blot和共定位分析，揭示了GEMIN4与SMN蛋白的直接相互作用，并解析了其在脊髓性肌萎缩症(SMA)病理中可能的分子机制。
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3. **标题**：*Dysregulation of GEMIN4 expression impairs cellular RNA splicing machinery*
**作者**：Feng W, et al.
**摘要**：研究通过RNA干扰和GEMIN4抗体检测发现，GEMIN4表达下调会导致剪接体功能异常，影响pre-mRNA加工，提示其在维持RNA代谢稳态中的重要作用。
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如需具体文献来源或更多信息，可进一步提供PMID或DOI编号。
The GEMIN4 antibody is a crucial tool for studying the GEMIN4 protein, a component of the survival of motor neuron (SMN) complex. This multi-protein complex, which includes SMN1. GEMIN2-8. and other partners, plays a central role in small nuclear ribonucleoprotein (snRNP) assembly, spliceosome formation, and pre-mRNA splicing. GEMIN4 directly interacts with GEMIN3 (a RNA helicase) and contributes to the structural integrity and functional coordination of the SMN complex. Research using GEMIN4 antibodies has linked this protein to spinal muscular atrophy (SMA), as SMN complex dysfunction impairs snRNP biogenesis, leading to motor neuron degeneration. Additionally, GEMIN4 is implicated in miRNA processing, DNA repair, and cellular stress responses.
GEMIN4 antibodies (commonly rabbit or mouse monoclonal/polyclonal) enable detection and localization of the protein via techniques like Western blotting, immunoprecipitation, and immunofluorescence. These reagents help investigate GEMIN4's expression patterns, interactions, and regulatory roles in diseases beyond SMA, including cancers (e.g., associations with PARK2 oncogene) and neurological disorders. Commercial GEMIN4 antibodies are typically validated using knockout cell lines or siRNA knockdown to confirm specificity. Studies utilizing these antibodies have advanced understanding of spliceosome mechanics, SMN complex dynamics, and potential therapeutic targets for spliceopathies.
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