| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TAMALIN |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GRASP |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GRASP抗体的3篇代表性文献及简要摘要：
1. **《GRASP55 regulates Golgi morphology and autophagy》**
作者：Wang et al. (2020)
摘要：研究通过GRASP55特异性抗体，揭示了该蛋白在维持高尔基体结构和调控自噬中的双重功能，发现其磷酸化状态影响细胞应激反应中的膜运输。
2. **《A role for GRASP65 in mitotic Golgi reorganization》**
作者：Tang et al. (2016)
摘要：利用GRASP65抗体进行免疫荧光和蛋白质互作分析，证明该蛋白通过调控高尔基体碎片化与重组，参与有丝分裂期间细胞器的动态重塑。
3. **《GRASP deficiency impairs immune cell function in zebrafish》**
作者：Chen et al. (2018)
摘要：通过基因敲除和GRASP抗体标记，阐明斑马鱼模型中GRASP蛋白缺失导致免疫细胞迁移缺陷，提示其在先天免疫中的保守作用。
注：以上文献为虚拟示例，实际研究中建议通过PubMed或Web of Science检索关键词“GRASP antibody”或“GORASP1/GORASP2”获取最新论文。
GRASP (Golgi ReAssembly Stacking Protein) antibodies are tools used to study the GRASP family of proteins, which play critical roles in Golgi apparatus structure and function. First identified in the early 2000s, GRASP proteins (GRASP55 and GRASP65 in mammals) are peripherally associated with Golgi membranes and are essential for maintaining its stacked cisternal architecture. They facilitate the tethering of incoming vesicles and mediate Golgi reassembly after mitosis or fragmentation during cellular stress. GRASP65 localizes to the cis-Golgi, while GRASP55 is found in the medial/trans regions, reflecting their distinct yet cooperative roles in membrane stacking and trafficking.
Antibodies targeting GRASP proteins are widely used in research to investigate Golgi dynamics, particularly during cell division, secretion, and stress responses. Studies using these antibodies have revealed GRASP's involvement in unconventional protein secretion pathways, where they bypass the classical ER-Golgi route, such as in the secretion of inflammatory cytokines like IL-1β. Dysregulation of GRASP proteins has been linked to diseases, including neurodegenerative disorders and cancers, where altered Golgi structure correlates with pathological secretion or signaling.
Recent work also highlights GRASP's non-canonical roles in autophagy, apoptosis, and cell adhesion, expanding its relevance beyond Golgi maintenance. GRASP antibodies thus serve as vital reagents for dissecting Golgi-related mechanisms in health and disease.
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