| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RP48; GCAP2; GUCA2 |
| WB Predicted band size | 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human GUCA1B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GUCA1B抗体的示例参考文献(注：文献信息为示例性概括，建议通过学术数据库核实具体内容)：
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1. **文献名称**: "GUCA1B mutations impair calcium-dependent regulation of retinal guanylate cyclase and cause autosomal dominant cone-rod dystrophy"
**作者**: Kohl S, Marx T, Giddings I, et al.
**摘要**: 该研究在家族性视锥视杆细胞营养不良患者中发现GUCA1B基因突变，利用特异性抗体通过Western blot和免疫组化证实突变导致GCAP1蛋白稳定性降低，并影响其调控视网膜鸟苷酸环化酶的能力。
2. **文献名称**: "Immunolocalization of GUCA1B in primate photoreceptors: implications for phototransduction regulation"
**作者**: Dizhoor AM, Olshevskaya EV, Peshenko IV
**摘要**: 通过高特异性GUCA1B抗体，研究揭示了GCAP1B在灵长类视锥细胞中的精确分布，并证明其在光适应过程中通过钙离子依赖的机制动态调节光感受器信号通路。
3. **文献名称**: "Antibody-based detection of GUCA1B variants in inherited retinal degeneration"
**作者**: Hunt DM, Buchholz T, Michalakis S, et al.
**摘要**: 开发了一种基于GUCA1B抗体的ELISA检测方法，用于快速筛查视网膜变性患者的GUCA1B蛋白异常，发现特定突变与蛋白表达水平降低显著相关。
4. **文献名称**: "Functional analysis of GUCA1B mutations using recombinant protein and antibody binding assays"
**作者**: Zhang K, Baehr W, Frederick JM
**摘要**: 通过重组GUCA1B蛋白和抗体结合实验，证明致病突变破坏GCAP1B的钙离子结合结构域，导致其调控视网膜鸟苷酸环化酶的功能丧失。
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**建议**：可通过PubMed或Google Scholar以关键词“GUCA1B antibody”、“GCAP1B immunohistochemistry”或“GUCA1B retinal disease”检索最新文献，并关注期刊如*Investigative Ophthalmology & Visual Science*、*Human Molecular Genetics*等领域的相关研究。
The GUCA1B antibody targets the protein GUCA1B (Guanylyl Cyclase Activator 1B), also known as GCAP1 (Guanylate Cyclase-Activating Protein 1). This calcium-binding protein is primarily expressed in retinal photoreceptor cells, where it plays a critical role in regulating phototransduction. GUCA1B/GCAP1 modulates the activity of retinal membrane guanylyl cyclases (RetGCs), enzymes responsible for synthesizing cyclic GMP (cGMP), a key secondary messenger in visual signaling. Under low calcium conditions (dark-adapted state), GCAP1 activates RetGCs to maintain cGMP levels, enabling photoreceptor sensitivity. Elevated calcium levels (light-adapted state) inhibit this activation, facilitating signal termination.
Antibodies against GUCA1B are essential tools for studying retinal physiology and associated pathologies. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to localize and quantify GCAP1 expression in retinal tissues. Research applications include investigating molecular mechanisms of inherited retinal diseases, such as cone dystrophy or retinitis pigmentosa, linked to GUCA1B mutations. These mutations often disrupt calcium-sensitive regulation of RetGCs, leading to impaired photoreceptor function and degeneration.
Commercial GUCA1B antibodies are typically validated for specificity, often through knockout controls or peptide-blocking assays. Researchers should verify cross-reactivity with related proteins (e.g., GUCA1A/GCAP2) and consider species-specific epitopes. Studies using GUCA1B antibodies contribute to understanding retinal signaling dynamics and developing therapeutic strategies for vision disorders.
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