| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | p240; GRIPE; GARNL1; TULIP1; RalGAPalpha1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human RALGAPA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RALGAPA1抗体的模拟参考文献示例(实际文献需通过学术数据库检索验证)：
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1. **文献名称**: "RALGAPA1 as a novel biomarker in hepatocellular carcinoma: Antibody validation and functional analysis"
**作者**: Chen L, et al.
**摘要**: 本研究通过Western blot和免疫组化验证了抗RALGAPA1抗体的特异性，发现其在肝癌组织中高表达，并与患者预后不良相关。进一步功能实验表明，RALGAPA1通过调控Ral GTP酶活性促进肿瘤细胞迁移。
2. **文献名称**: "Development of a monoclonal antibody targeting RALGAPA1 for studying Ral signaling pathways"
**作者**: Sato K, et al.
**摘要**: 报道了一种高特异性抗RALGAPA1单克隆抗体的开发，该抗体成功用于检测内源性RALGAPA1蛋白表达，并揭示其在RalA/B信号通路中的负调控作用，为研究Ral相关肿瘤机制提供工具。
3. **文献名称**: "Proteomic screening identifies RALGAPA1-interacting proteins using co-immunoprecipitation assays"
**作者**: Müller S, et al.
**摘要**: 利用抗RALGAPA1抗体进行免疫共沉淀实验，结合质谱分析发现其与多个细胞骨架调控蛋白相互作用，提示RALGAPA1可能在细胞极性和分裂中发挥功能。
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**注**：以上内容为模拟生成，实际文献请通过PubMed、Google Scholar或Web of Science以关键词“RALGAPA1 antibody”或“RALGAPA1 + (Western blot/immunoprecipitation)”检索。
The RALGAPA1 antibody is a tool used to detect Ral GTPase-activating protein alpha subunit 1 (RALGAPA1), a key regulatory component of the RalGAP complex. RALGAPA1. along with its beta subunit (RALGAPB), forms the RalGAP enzyme, which negatively regulates Ral GTPases (RalA and RalB) by accelerating their GTP hydrolysis, thereby inactivating these signaling molecules. Ral GTPases are downstream effectors of the Ras oncoprotein and play roles in cell proliferation, vesicle trafficking, and tumorigenesis. Dysregulation of Ral signaling is implicated in cancers, making RALGAPA1 a potential biomarker or therapeutic target.
The RALGAPA1 antibody is commonly used in research to study RalGAP complex expression, localization, and function in cellular models or tissues. It aids in elucidating RALGAPA1's role in suppressing Ral-driven pathways and its crosstalk with other oncogenic signals, such as Ras or PI3K/AKT. Applications include Western blotting, immunohistochemistry, and immunofluorescence. Commercial antibodies are typically raised against specific epitopes (e.g., human RALGAPA1 N- or C-terminal regions) and validated for specificity across species (human, mouse, rat).
Research using this antibody has linked RALGAPA1 loss or mutations to enhanced Ral activity in cancers like pancreatic adenocarcinoma and melanoma. Its study also extends to non-cancer contexts, such as metabolic regulation and neuronal development, highlighting its broad biological relevance.
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