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Human gonadotropin-releasing hormone,GnRH ELISA Kit

  • 中文名： 人促性腺激素释放激素(GnRH)ELISA KIT
货号: IPD-E06880h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人促性腺激素释放激素(GnRH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GNRH1含量。GNRH1是重要靶点。它是促性腺激素释放激素基因。其背景上，在生殖内分泌调节中有关键作用。研究机制方面，主要是通过调控促性腺激素分泌，影响性腺发育和功能，对生殖系统疾病治疗和生殖调控研究意义重大。试剂盒检测范围为2.5 pg/ml-100 pg/ml.，适用于生命科学领域的研究，例如探究生殖生理机制、评估内分泌干扰物的效应、分析动物模型生殖轴功能异常等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    GNRH1 ELISA Kit; GNRH ELISA Kit; GRH ELISA Kit; LHRHProgonadoliberin-1 ELISA Kit; Progonadoliberin I) [Cleaved into: Gonadoliberin-1 ELISA Kit; Gonadoliberin I ELISA Kit; Gonadorelin ELISA Kit; Gonadotropin-releasing hormone I ELISA Kit; GnRH-I ELISA Kit; Luliberin I ELISA Kit; Luteinizing hormone-releasing hormone I ELISA Kit; LH-RH I); GnRH-associated peptide 1 ELISA Kit; GnRH-associated peptide I)] ELISA Kit
  • 缩写：
    GNRH1
  • Uniprot No.：
    P01148
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    2.5 pg/ml-100 pg/ml.
  • 灵敏度：
    0.625 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<15%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    92


    Range %

    86-98


    1:2

    Average %

    97


    Range %

    92-106


    1:4

    Average %

    85


    Range %

    81-88


    1:8

    Average %

    89


    Range %

    83-96


  • 回收率：

    The recovery of human GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    91

    87-98


    EDTA plasma (n=4)

    93

    88-99


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    100

    2.346

    2.489

    2.418

    2.378


    50

    1.725

    1.832

    1.779

    1.739


    25

    0.968

    1.050

    1.009

    0.969


    6.5

    0.352

    0.350

    0.351

    0.311


    2.5

    0.176

    0.179

    0.178

    0.138


    0

    0.039

    0.040

    0.040




  • 货期：
    3-5 working days

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