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产品描述：人糖化血红蛋白A1c(GHbA1c)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测lysatesforRBC样本中的GHbA1c含量。GHbA1c即糖化血红蛋白，它反映过去2-3个月平均血糖水平。其背景在于可用于糖尿病的诊断、病情监测。研究机制方面，血糖与血红蛋白结合形成糖化血红蛋白，通过检测其水平能评估血糖控制情况，为治疗方案调整提供依据。试剂盒检测范围为0.012 ng/mL-50 ng/mL，适用于科研领域对红细胞裂解液样本中GHbA1c含量的精准检测；在糖尿病病理机制研究、降糖药物开发及红细胞代谢分析等方向具有重要科研价值；适用于体外实验中对红细胞糖化程度的动态监测、抗糖尿病化合物筛选以及代谢综合征相关基础研究，为探索糖代谢异常与靶器官损伤关联性提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：GHbA1c
种属：Homo sapiens (Human)
样本类型：lysates for RBC
检测范围：0.012 ng/mL-50 ng/mL
灵敏度：0.012 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 500.2700.2730.272 12.50.3670.3780.373 3.1250.4630.4890.476 0.780.5600.5980.579 0.1950.6920.6720.682 0.0491.3841.3681.376 0.0121.9941.9541.974 02.3732.3992.386 | ||||||
本试剂盒所含材料：
A 96-well Assay plate --The 96-well plate has been pre-coated with GHbA1c antibody.
Standard (10 x concentrate) (1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-conjugated GHbA1c (100 x concentrate) (1 x 60 μl)
HRP-avidin (100 x concentrate) (1 x 120 μl) --Catalyze the TMB solution, eliciting a chromogemic reaction.
Biotin-conjugate Diluent (1 x 10 ml) --Dilute the Biotin-conjugated GHbA1c
HRP-avidin Diluent (1 x 20 ml) --Dilute the HRP-avidin
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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