| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ODS1 |
| WB Predicted band size | 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human ME2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ME2抗体的示例参考文献(注：以下为假设性示例，实际文献需通过学术数据库检索确认)：
1. **标题**：Development of a Monoclonal Antibody Targeting Mitochondrial Malic Enzyme 2 (ME2) for Cancer Research
**作者**：Smith J, et al.
**摘要**：研究团队开发了一种高特异性ME2单克隆抗体，通过Western blot和免疫组化验证其在多种癌细胞系中的靶向性，用于探究ME2在肿瘤代谢异常中的表达调控机制。
2. **标题**：ME2 Antibody-Based Detection of Metabolic Reprogramming in Hepatocellular Carcinoma
**作者**：Zhang L, et al.
**摘要**：本文利用商业化ME2多克隆抗体，结合质谱分析验证其特异性，揭示了肝癌组织中ME2表达上调与糖脂代谢重编程的相关性，为癌症诊断提供潜在标志物。
3. **标题**：Role of ME2 in Adipocyte Differentiation: Insights from Antibody-Mediated Protein Localization
**作者**：Wang Y, et al.
**摘要**：通过抗ME2抗体的免疫荧光技术，研究发现ME2在脂肪细胞线粒体中的动态定位变化，提示其参与分化过程中的氧化还原平衡调控。
4. **标题**：Validation of Cross-Species Reactivity of Anti-ME2 Antibodies in Preclinical Models
**作者**：Brown K, et al.
**摘要**：系统评估了多种ME2抗体的种属交叉反应性(人/小鼠/大鼠)，为代谢疾病动物模型中靶向ME2的实验设计提供抗体选择依据。
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**建议**：实际文献可通过PubMed或Google Scholar以关键词“ME2 antibody”、“malic enzyme 2 immunoassay”检索，优先选择近5年发表、高被引论文，并关注《Cell Metabolism》《Oncogene》等期刊。部分抗体厂商(如CST、Abcam)官网的技术文档也可能提供引用文献。
ME2 antibody targets malic enzyme 2 (ME2), a mitochondrial enzyme critical for cellular energy metabolism. ME2 catalyzes the oxidative decarboxylation of malate to pyruvate, coupled with NAD+ reduction to NADH, playing a key role in the tricarboxylic acid (TCA) cycle and linking glycolysis with mitochondrial respiration. This enzyme also contributes to biosynthetic pathways by generating precursors for lipid and nucleotide synthesis. Dysregulation of ME2 has been implicated in metabolic disorders, cancer progression, and oxidative stress responses. In cancer, ME2 overexpression is associated with enhanced tumor growth, metastasis, and chemoresistance, likely due to its role in maintaining redox balance and supporting rapid proliferation.
ME2 antibodies are essential tools for detecting ME2 protein levels in research applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They aid in studying ME2's tissue-specific expression, subcellular localization, and interactions in disease models. Validated antibodies help elucidate ME2's regulatory mechanisms in metabolic reprogramming (e.g., the Warburg effect) and its potential as a therapeutic target. Specificity is often confirmed using knockout cell lines or recombinant protein controls. Research using ME2 antibodies continues to explore its dual role in energy production and antioxidant defense, offering insights into targeting metabolic vulnerabilities in cancer and other diseases.
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