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产品描述：人γ谷氨酰转移酶(GGT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GGT1含量。GGT1是一种重要靶点。它参与谷胱甘肽代谢，在细胞抗氧化防御等生理过程中发挥作用。研究发现其异常表达与多种疾病如肿瘤等密切相关，科学家正深入探究其作用机制，期望基于此开发新的诊断标志物和治疗靶点。试剂盒检测范围为0.78 mU/mL-50 mU/mL，适用于探索肝脏功能异常模型、评估药物肝毒性或研究氧化应激相关机制等科研领域；为体外实验或动物模型研究提供可靠工具；可满足基础研究或转化医学中对GGT活性动态监测的需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GGT1; GGT; Glutathione hydrolase 1 proenzyme; Gamma-glutamyltransferase 1; Gamma-glutamyltranspeptidase 1; GGT 1; Leukotriene-C4 hydrolase; CD antigen CD224
缩写：GGT1
Uniprot No.：P19440
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 mU/mL-50 mU/mL
灵敏度：0.195 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GGT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
| 1:2 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 100-112 | ||||||
回收率：
| The recovery of human GGT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 88-98 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| mU/mlOD1OD2AverageCorrected501.9912.0692.0301.846251.4381.4941.4661.28212.50.9961.0321.0140.8306.250.7200.7110.7160.5323.120.4840.4770.4810.2971.560.3480.3380.3430.1590.780.2490.2650.2570.07300.1810.1860.184 | |||||||
货期：3-5 working days
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