| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | AS; HLAB; B-4901 |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human HLA-B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于HLA-B抗体的代表性文献概览：
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1. **文献名称**：*"HLA-specific antibodies in transplantation: Mechanisms and clinical relevance"*
**作者**：El-Awar, N., et al.
**摘要**：探讨HLA-B抗体在器官移植中的临床意义，发现HLA-B抗体与肾移植后的急性排斥反应及移植物存活率降低相关，强调术前抗体筛查的重要性。
2. **文献名称**：*"Impact of HLA-B antibodies on platelet transfusion refractoriness"*
**作者**：Taaning, E., et al.
**摘要**：研究HLA-B抗体在血小板输注无效中的作用，证实患者体内HLA-B抗体的存在显著降低血小板输注后的恢复率，需通过配型策略改善疗效。
3. **文献名称**：*"HLA-B antibody detection: Comparison of Luminex and flow cytometry methods"*
**作者**：Lucas, D.P., et al.
**摘要**：比较Luminex单抗原微珠法与流式细胞术检测HLA-B抗体的敏感性和特异性，指出Luminex技术更适用于低滴度抗体的精准识别。
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**备注**：以上文献为示例，实际引用时请核对原文准确性及最新研究进展。
HLA-B antibodies target human leukocyte antigen (HLA)-B molecules, a class of major histocompatibility complex (MHC) proteins critical for immune regulation. Encoded by the HLA-B gene on chromosome 6. HLA-B molecules are expressed on most nucleated cells and present peptide antigens to CD8+ T cells, triggering immune responses. Their high genetic polymorphism—over 4.000 HLA-B alleles identified—creates significant diversity across populations, complicating tissue compatibility in transplants.
These antibodies typically arise from exposure to non-self HLA-B antigens through pregnancy, blood transfusions, or prior transplants. In transplantation, preformed or post-transplant HLA-B antibodies can mediate hyperacute or chronic rejection by binding donor cells, activating complement pathways, and promoting endothelial injury. They are a key focus in organ allocation protocols, necessitating crossmatch testing and donor-recipient HLA matching to minimize rejection risks.
HLA-B antibodies are also implicated in autoimmune conditions. For example, HLA-B*27 variants are strongly associated with ankylosing spondylitis, though the mechanism linking antibodies to pathogenesis remains unclear. Additionally, they contribute to platelet transfusion refractoriness by targeting HLA-B on donor platelets.
Detection methods include solid-phase assays (e.g., Luminex) and cell-based crossmatches. Clinical management may involve immunosuppression, plasmapheresis, or tailored donor selection. Understanding HLA-B antibody dynamics is essential for improving transplant outcomes and managing immune-related disorders.
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