首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠神经胶质纤维酸性蛋白(GFAP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GFAP含量。GFAP即胶质纤维酸性蛋白，是星形胶质细胞的中间丝蛋白。其背景在于它在中枢神经系统发育、维持细胞结构等方面起重要作用。研究机制上，常作为星形胶质细胞活化标志物，用于神经系统疾病如脑损伤、神经退行性疾病等的病理研究。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于脑损伤模型评估、神经退行机制研究、胶质细胞活化分析及药物神经保护效应筛选等科研场景，组织匀浆检测功能可拓展至脑区特异性 GFAP 表达分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gfap ELISA Kit; Glial fibrillary acidic protein ELISA Kit; GFAP ELISA Kit
缩写：GFAP
Uniprot No.：P47819
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GFAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 94-109 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 89-104 | |||||
回收率：
| The recovery of rat GFAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 98-108 | ||||
| EDTA plasma (n=4) | 98 | 94-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.8042.9252.8652.66425002.1232.2012.1621.96112501.5291.5071.5181.3176250.9360.9140.9250.7243120.5900.5760.5830.3821560.4240.4320.4280.227780.3090.3000.3050.10400.2040.1980.201 | ||||||
货期：3-5 working days
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