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Human FAD-linked sulfhydryl oxidase ALR(GFER) ELISA kit

  • 中文名： 人FAD链接巯基氧化酶ALR(GFER)ELISA kit
货号: IPD-EL009370HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人FAD链接巯基氧化酶ALR(GFER)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GFER含量。GFER，全称为生长因子、肝脏再生增强因子，是一种在人类中由GFER基因编码的蛋白质。它是一种自分泌保肝生长因子，对肝脏再生具有重要作用。GFER的研究机制涉及其在氧化磷酸化、维持线粒体基因组以及细胞分裂周期中的功能，同时与COP9和BNIP等蛋白相互作用。试剂盒检测范围为1.56 pg/ml-100 pg/ml，本试剂盒适用于基础科研中探究GFER在肝脏病理模型、代谢性疾病或神经退行性疾病中的作用机制，也可用于药物开发中评估化合物对线粒体功能及细胞氧化还原状态的调控效果。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    ALR_HUMAN ELISA Kit; Augmenter of liver regeneration ELISA Kit; ERV1 ELISA Kit; ERV1 homolog ELISA Kit; Erv1 like growth factor ELISA Kit; FAD-linked sulfhydryl oxidase ALR ELISA Kit; GFER ELISA Kit; Growth factor augmenter of liver regeneration ELISA Kit; Growth factor erv1 like ELISA Kit; Hepatic regenerative stimulation substance ELISA Kit; Hepatopoietin ELISA Kit; Hepatopoietin protein ELISA Kit; hERV1 ELISA Kit; HPO ELISA Kit; HPO1 ELISA Kit; HPO2 ELISA Kit; HSS ELISA Kit
  • 缩写：
    GFER
  • Uniprot No.：
    P55789
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 pg/ml-100 pg/ml
  • 灵敏度：
    0.39 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human GFER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:10

    Average %

    104


    Range %

    99-109


    1:20

    Average %

    95


    Range %

    88-101


    1:40

    Average %

    89


    Range %

    84-95


    1:80

    Average %

    98


    Range %

    91-104


  • 回收率：

    The recovery of human GFER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    96

    89-103


    EDTA plasma (n=4)

    91

    87-96


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    100

    2.344

    2.434

    2.389

    2.251


    50

    1.708

    1.667

    1.687

    1.549


    25

    1.269

    1.228

    1.249

    1.111


    12.5

    0.899

    0.929

    0.914

    0.776


    6.25

    0.571

    0.581

    0.576

    0.438


    3.12

    0.384

    0.363

    0.373

    0.236


    1.56

    0.265

    0.274

    0.270

    0.132


    0

    0.141

    0.135

    0.138




  • 货期：
    3-5 working days

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