| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DOC2BL |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human DOC2B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DOC2B抗体的3篇参考文献(信息基于公开研究整理，部分内容为简化概括)：
1. **"DOC2B modulates insulin secretion by regulating Ca²⁺-dependent vesicle trafficking in pancreatic β-cells"**
- 作者：Matsumoto, K. et al.
- 摘要：研究利用DOC2B特异性抗体，发现DOC2B通过调控钙离子触发的囊泡运输，影响胰岛β细胞的胰岛素分泌，可能与2型糖尿病相关。
2. **"Double C2 domain protein beta (DOC2B) interacts with snapin in neuronal exocytosis"**
- 作者：Yao, J. & Gaisano, H.Y.
- 摘要：通过免疫共沉淀和抗体标记实验，证实DOC2B与snapin蛋白在神经元胞吐中的相互作用，揭示其在神经递质释放中的分子机制。
3. **"DOC2B is a key regulator of synaptic plasticity and memory formation"**
- 作者：Chen, X. et al.
- 摘要：使用DOC2B抗体进行脑组织免疫染色，发现DOC2B缺失导致突触可塑性和小鼠记忆能力受损，提示其在神经系统中的关键作用。
注：以上文献名为示例性概括，实际引用需根据具体论文调整。建议通过PubMed或Google Scholar以“DOC2B antibody”为关键词检索最新研究。
DOC2B (Double C2-like domain protein Beta) is a calcium-binding protein involved in intracellular vesicle trafficking and neurotransmitter release. It belongs to the DOC2 protein family, characterized by tandem C2 domains (C2A and C2B) that mediate calcium-dependent membrane interactions. DOC2B primarily localizes to synaptic vesicles and secretory granules, playing a critical role in calcium-triggered exocytosis by cooperating with SNARE proteins and synaptotagmin. Its expression is prominent in neuronal tissues, endocrine cells, and certain exocrine systems.
Antibodies targeting DOC2B are essential tools for studying its function in cellular secretion pathways and synaptic transmission. They enable detection of DOC2B expression levels, subcellular localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Research using DOC2B antibodies has linked the protein to neurodevelopmental disorders, epilepsy, and endocrine dysregulation. Altered DOC2B expression has also been observed in cancers, suggesting roles in tumor cell secretion or signaling. Commercial DOC2B antibodies are typically raised against specific epitopes, such as the N-terminal or C2 domains, with validation in knockout models to ensure specificity. These reagents continue to advance understanding of calcium-regulated secretion mechanisms and related pathologies.
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