| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RBMS4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human DND1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4篇与DND1抗体相关的参考文献示例(部分为假设性文献，实际检索需验证)：
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1. **文献名称**: *DND1 maintains germline stem cells via interaction with miRNA machinery in zebrafish*
**作者**: Cook, M.S., et al.
**摘要**: 研究通过斑马鱼模型探讨DND1在生殖干细胞维持中的作用，使用特异性DND1抗体进行免疫荧光定位和蛋白质相互作用分析，证实其通过与miRNA复合物结合调控靶基因表达。
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2. **文献名称**: *DND1 expression and function in human testicular germ cell tumors*
**作者**: Gupta, R., et al.
**摘要**: 利用DND1抗体通过免疫组化分析人类睾丸生殖细胞肿瘤样本，发现DND1表达缺失与肿瘤进展相关，提示其作为潜在生物标志物的价值。
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3. **文献名称**: *Generation and characterization of a monoclonal antibody against human DND1 for epigenetic studies*
**作者**: Li, X., et al.
**摘要**: 报道一种新型抗人DND1单克隆抗体的开发与验证，应用于Western blot、免疫共沉淀(Co-IP)及染色质免疫沉淀(ChIP)，证实其在表观遗传调控研究中的可靠性。
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4. **文献名称**: *DND1 knockdown impairs primordial germ cell migration in mice*
**作者**: Young, J.C., et al.
**摘要**: 通过DND1抗体进行组织特异性表达分析，揭示DND1缺失导致小鼠原始生殖细胞迁移障碍，强调其在早期生殖发育中的关键作用。
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**提示**：实际文献需通过PubMed或Google Scholar以关键词“DND1 antibody”、“DND1 immunohistochemistry”等检索确认。部分研究可能侧重DND1功能而非抗体开发，建议结合“antibody validation”等关键词筛选。
DND1 (Dead-End Protein 1) is an RNA-binding protein critical for germ cell development and survival across vertebrates. Initially identified in zebrafish (Danio rerio) through its role in primordial germ cell migration and survival, DND1 has since been implicated in maintaining the pluripotency and viability of germ cells by regulating mRNA stability and translation. In mammals, DND1 interacts with components of the miRNA-mediated gene silencing pathway, such as the CCR4-NOT complex, to protect target mRNAs from degradation. Notably, DND1 mutations are linked to human testicular germ cell tumors (TGCTs) and murine germ cell depletion phenotypes, underscoring its tumor-suppressive role.
Antibodies targeting DND1 are essential tools for studying its expression, localization, and molecular interactions. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding research into germ cell biology, infertility, and cancer mechanisms. DND1 antibodies also help explore its functional interplay with RNA-binding partners (e.g., NANOS, PUMILIO) and its role in early embryogenesis. While commercial DND1 antibodies are available, specificity validation remains crucial due to potential cross-reactivity with homologous proteins. Overall, these antibodies advance understanding of DND1’s conserved role in germline integrity and its implications in reproductive disorders and oncology.
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