Rabbit Polyclonal DCAF13 Antibody

The DCAF13 (DDB1- and CUL4-associated factor 13) antibody is a research tool used to study the role of the DCAF13 protein in cellular processes. DCAF13 is a substrate-recognition component of the CRL4 (Cullin 4-RING Ligase 4) E3 ubiquitin ligase complex, which mediates targeted protein ubiquitination and degradation via the proteasome. This protein is critical for regulating processes like DNA damage repair, cell cycle progression, and chromatin remodeling. DCAF13 interacts with DDB1 to recruit specific substrates for CRL4-dependent ubiquitination, impacting genome stability and cellular homeostasis. Dysregulation of DCAF13 has been implicated in cancer, developmental disorders, and other diseases linked to proteostatic imbalances. Antibodies targeting DCAF13 are utilized in techniques such as Western blotting, immunofluorescence, and co-immunoprecipitation to explore its expression, localization, and interactions in various biological contexts. Researchers also employ these antibodies to investigate DCAF13's role in CRL4 complex assembly, substrate specificity, and its potential as a therapeutic target. Studies have highlighted its involvement in pathways like p53 signaling and ribosome biogenesis, underscoring its multifaceted regulatory functions. Validation of DCAF13 antibodies includes specificity checks using knockout cell lines or siRNA knockdown to ensure reliable detection in experimental models.