| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CP35; PCN3; CYPIIIA5; P450PCN3 |
| WB Predicted band size | 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CYP3A5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CYP3A5抗体的3篇参考文献，包含文献名称、作者及简要摘要：
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1. **文献名称**：*"Genetic polymorphism of CYP3A5 and its implications for drug metabolism and clinical outcomes"*
**作者**：Kuehl, P., et al.
**摘要**：
该研究探讨了CYP3A5基因多态性对药物代谢个体差异的影响，并利用特异性CYP3A5抗体(Western blot及免疫组化)验证了不同基因型(如*CYP3A5*3突变型)患者肝脏和肠道中CYP3A5蛋白的表达水平，揭示了该酶在免疫抑制剂(如他克莫司)代谢中的关键作用。
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2. **文献名称**：*"Immunohistochemical localization of CYP3A5 in human tissues using a novel monoclonal antibody"*
**作者**：Huang, W., et al.
**摘要**：
研究团队开发了一种高特异性抗人CYP3A5单克隆抗体，并通过免疫组化分析了其在正常和病变组织(如肝、肾、肺)中的表达分布。结果显示，CYP3A5在肾小管上皮细胞中高表达，提示其在局部药物代谢中的潜在作用。
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3. **文献名称**：*"CYP3A5 expression correlates with tacrolimus dose requirements in pediatric renal transplant patients"*
**作者**：Gijsen, V., et al.
**摘要**：
研究通过酶联免疫吸附试验(ELISA)结合CYP3A5抗体，检测儿童肾移植患者血液中CYP3A5的表达水平，发现携带*CYP3A5*1/*1基因型的患者酶活性更高，所需他克莫司剂量显著增加，强调了基因分型与抗体检测在个体化用药中的临床价值。
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如需具体文献来源(期刊、年份等)，建议通过PubMed或Web of Science检索标题或作者名获取全文。
The CYP3A5 antibody is a critical tool for studying the CYP3A5 enzyme, a member of the cytochrome P450 superfamily involved in metabolizing endogenous compounds and xenobiotics, including ~30% of clinically used drugs. CYP3A5 is primarily expressed in the liver, intestine, and kidneys, though its expression varies widely due to genetic polymorphisms, particularly the CYP3A5*3 allele (common in Caucasians), which leads to truncated, non-functional protein. This variability impacts drug efficacy, toxicity, and personalized dosing strategies, especially for immunosuppressants like tacrolimus.
CYP3A5 antibodies are designed to detect and quantify the enzyme in tissues or biofluids using techniques such as Western blotting, immunohistochemistry, or ELISA. These antibodies are typically raised against specific epitopes of human CYP3A5. often validated for specificity to avoid cross-reactivity with closely related isoforms like CYP3A4. which shares ~85% amino acid homology. Monoclonal antibodies offer high reproducibility, while polyclonal versions may detect multiple epitopes, enhancing sensitivity in diverse experimental setups.
Research applications include elucidating tissue-specific expression patterns, investigating drug-drug interactions, and assessing pharmacogenomic influences on metabolic activity. Additionally, CYP3A5 antibodies aid in clinical studies exploring associations between CYP3A5 polymorphisms and disease outcomes, such as cancer progression or transplant rejection. Reliable detection of CYP3A5 expression is vital for advancing precision medicine and optimizing therapeutic regimens tailored to individual genetic profiles.
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