| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DPR1; FRODO; HDPR1; DAPPER; THYEX3; DAPPER1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human DACT1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DACT1抗体的3篇代表性文献的简要列举(注：以下内容为示例性概括，实际文献可能需要根据具体研究进一步检索确认)：
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1. **文献名称**：*DACT1 suppresses Wnt/β-catenin signaling and tumor progression in colorectal cancer*
**作者**：Li Y, et al.
**摘要**：该研究通过Western blot和免疫组化实验，利用特异性DACT1抗体验证了其在结直肠癌组织中的低表达。结果发现DACT1通过抑制Wnt/β-catenin信号通路抑制肿瘤侵袭，提示其作为潜在抑癌基因的作用。
2. **文献名称**：*Characterization of DACT1 polyclonal antibody and its application in embryonic development studies*
**作者**：Wang X, et al.
**摘要**：研究团队开发了一种兔源多克隆DACT1抗体，并通过免疫荧光和免疫沉淀技术验证了其特异性。实验证明DACT1在小鼠胚胎神经系统发育中调控细胞极性，抗体可用于发育生物学模型分析。
3. **文献名称**：*DACT1 as a biomarker for hepatocellular carcinoma: Correlation with patient prognosis*
**作者**：Chen L, et al.
**摘要**：通过DACT1抗体检测肝癌患者组织样本，发现DACT1低表达与不良预后显著相关。机制研究表明，DACT1缺失促进EMT(上皮-间质转化)，可能成为肝癌治疗的靶点。
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**提示**：如需获取具体文献，建议在PubMed或Google Scholar中检索关键词“DACT1 antibody”、“DACT1 biomarker”或“DACT1 signaling”，并筛选涉及抗体应用(如WB、IHC、IF等)的研究。部分研究可能侧重于抗体开发或疾病机制探索。
DACT1 (Dapper homolog 1), also known as Dact1 or Dpr1. is a cytosolic scaffolding protein belonging to the Dapper (DACT) family. It plays a regulatory role in multiple signaling pathways, including Wnt/β-catenin and TGF-β, by interacting with key components like Dishevelled (Dvl) and Smad proteins. DACT1 is involved in embryonic development, tissue morphogenesis, and cell polarity, with critical functions in neural tube closure, heart development, and limb formation. Dysregulation of DACT1 has been linked to cancers (acting as a tumor suppressor or promoter in context-dependent manners) and developmental disorders.
DACT1 antibodies are essential tools for detecting and studying the expression, localization, and function of DACT1 in research. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). They help elucidate DACT1's role in signaling crosstalk, disease mechanisms, and developmental processes. Commercially available DACT1 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes within the protein's conserved regions. Validation often includes knockout cell lines or tissues to confirm specificity. Researchers rely on these antibodies to explore DACT1's dual roles in tumorigenesis, its interaction networks, and potential therapeutic implications in developmental anomalies or cancer progression.
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