首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠纤维蛋白原样蛋白1(FGL1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGL1含量。FGL1是一种在肿瘤细胞和肝细胞中表达的免疫检查点分子，其表达在多种肿瘤中上调，与抗PD-L1/PD-1治疗的耐药性和预后不良有关。研究揭示FGL1通过作为LAG-3的主要配体抑制T细胞活化，其乙酰化修饰和泛素化降解调控肿瘤免疫逃逸。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中探究肝脏代谢功能、免疫调节机制、肿瘤微环境等领域的FGL1动态表达研究，为动物模型构建、药物干预效果评估及分子机制解析提供可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fgl1 ELISA Kit; Mfire1 ELISA Kit; Fibrinogen-like protein 1 ELISA Kit; Fibrinogen-related protein 1 ELISA Kit; Mfrep-1 ELISA Kit; Liver fibrinogen-related protein-1 ELISA Kit; Mfire-1 ELISA Kit
缩写：FGL1
Uniprot No.：Q71KU9
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:200 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:400 | Average % | 83 | ||||
| Range % | 90-96 | |||||
| 1:800 | Average % | 87 | ||||
| Range % | 84-90 | |||||
回收率：
| The recovery of mouse FGL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 100-108 | ||||
| EDTA plasma (n=4) | 107 | 104-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average20000.0760.0790.07810000.1350.1460.1415000.2060.2200.2132500.3620.3790.3711250.5930.5860.59062.50.9010.9320.91731.251.2131.2361.22502.0542.0982.076 | ||||||
货期：3-5 working days
×
