首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠成纤维细胞生长因子5(FGF5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGF5含量。FGF5是一个重要靶点。它属于成纤维细胞生长因子家族，在毛囊发育、毛发周期调控等生理过程有重要作用。研究发现其异常表达可能与脱发等毛发问题相关，通过调控FGF5的表达水平或活性，有望为治疗毛发疾病带来新策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，可广泛应用于毛发生长调控机制、基因功能验证、疾病动物模型构建等基础研究领域，也可用于评估药物或基因干预对FGF5表达的影响，为毛发相关疾病研究提供可靠的科研工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fgf5 ELISA kit; Fgf-5Fibroblast growth factor 5 ELISA kit; FGF-5 ELISA kit; Heparin-binding growth factor 5 ELISA kit; HBGF-5 ELISA kit
缩写：FGF5
Uniprot No.：P15656
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 92-103 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 86-99 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 88-94 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 96-106 | ||||||
回收率：
| The recovery of mouse FGF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 87-94 | |||||
| EDTA plasma (n=4) | 106 | 101-111 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.5552.6332.5942.48510002.1422.2582.2002.0915001.5671.6141.5911.4822501.0331.1021.0680.9591250.7250.7540.7400.63162.50.4710.4450.4580.34931.250.2140.2210.2180.10900.1050.1120.109 | |||||||
货期：3-5 working days
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