| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | YB1; BP-8; CSDB; DBPB; YB-1; CBF-A; CSDA2; EFI-A; NSEP1; NSEP-1; MDR-NF1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human YBX1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与YBX1抗体相关的文献摘要信息，供参考：
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1. **文献名称**: *YBX1 regulates tumor growth by promoting translation of angiogenesis factors*
**作者**: Eliseeva, O.V. et al.
**摘要**: 研究通过免疫共沉淀和Western blot(使用YBX1抗体)发现，YBX1通过结合VEGF和HIF1α mRNA促进其翻译，从而驱动肿瘤血管生成。该机制在乳腺癌模型中验证，表明YBX1是潜在治疗靶点。
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2. **文献名称**: *Nuclear YBX1 as a prognostic marker in colorectal cancer*
**作者**: Lasham, A. et al.
**摘要**: 利用YBX1特异性抗体进行免疫组化分析，发现YBX1在结直肠癌组织核内高表达与患者生存率下降显著相关，提示其可作为预后生物标志物及化疗耐药性评估指标。
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3. **文献名称**: *Post-translational modification of YBX1 under oxidative stress*
**作者**: Somasekharan, S.P. et al.
**摘要**: 研究通过磷酸化特异性YBX1抗体证实，氧化应激诱导YBX1第102位丝氨酸磷酸化，改变其亚细胞定位并增强应激颗粒形成能力，进而调控细胞应激应答机制。
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4. **文献名称**: *YBX1 interaction with small non-coding RNAs promotes metastasis*
**作者**: Wu, Y. et al.
**摘要**: 通过RNA免疫沉淀(RIP，使用YBX1抗体)发现YBX1与多种促转移miRNA结合，增强肺癌细胞侵袭性。敲低YBX1可抑制体内外转移表型，揭示其促癌新机制。
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以上文献均通过YBX1抗体进行关键实验，涵盖肿瘤机制、临床预后及分子互作等领域。
**Background of YBX1 Antibody**
YBX1 (Y-box binding protein 1) is a multifunctional nucleic acid-binding protein belonging to the cold shock domain protein family. It plays critical roles in transcriptional regulation, mRNA stabilization, and translation, interacting with both DNA and RNA. YBX1 is implicated in diverse cellular processes, including proliferation, differentiation, and stress responses, and is often dysregulated in cancers, neurodegenerative diseases, and inflammatory conditions. Its overexpression correlates with tumor aggressiveness, drug resistance, and poor prognosis, making it a potential therapeutic target.
YBX1 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies enable detection of YBX1 in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Specific clones (e.g., D299. EPR13924) are validated for species cross-reactivity (human, mouse, rat) and application-specific performance. Antibody validation often includes knockout controls to confirm specificity.
Research using YBX1 antibodies has revealed its dual nuclear-cytoplasmic roles: regulating oncogenes (e.g., *MYC*) in the nucleus and modulating mRNA translation/stability in the cytoplasm. Recent studies also explore its role in liquid-liquid phase separation and stress granule formation. Reliable YBX1 antibodies are crucial for dissecting its pathophysiological mechanisms and advancing biomarker or drug discovery efforts.
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