首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠成纤维细胞生长因子23(FGF23)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FGF23含量。FGF23 即成纤维细胞生长因子 23，是一种主要由骨细胞产生的激素。它在调节体内磷代谢和维生素 D 稳态中起关键作用。其研究机制主要围绕与受体及共受体结合，调控肾脏对磷的重吸收和活性维生素 D 合成，异常表达与多种骨代谢疾病相关。试剂盒检测范围为1.56 pg/mL-100 pg/mL，主要应用于骨代谢研究、肾脏疾病机制探索、内分泌紊乱模型构建等科研领域，特别适用于矿物质代谢相关动物模型的血清标志物分析、体外细胞因子分泌检测以及骨/肾组织样本的分子水平研究，为深入解析FGF23在生理病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fgf23Fibroblast growth factor 23 ELISA Kit; FGF-23 ELISA Kit
缩写：FGF23
Uniprot No.：Q8VI82
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat FGF23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:40 | Average % | 97 | ||||
| Range % | 91-101 | |||||
| 1:80 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:160 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of rat FGF23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 94 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.5322.4312.4822.291502.0892.0122.0511.860251.6141.5731.5941.40312.51.0981.0271.0630.8726.250.6580.7050.6820.4913.120.5270.5530.5400.3491.560.3070.3160.3120.12100.1840.1970.191 | ||||||
货期：3-5 working days
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