首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠凋亡相关因子(FAS/CD95)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FAS含量。FAS（Fas/FasL系统）是细胞凋亡信号转导途径的主要系统，由Fas（APO-1/CD95）和其配体FasL组成。Fas/FasL系统通过死亡受体与配体结合，激活下游的Caspase-8和Caspase-3，进而引发细胞凋亡。该系统在免疫监视、肿瘤发生和免疫逃逸中发挥重要作用。试剂盒检测范围为0.78 ng/mL-50 ng/mL，FAS/CD95作为细胞表面凋亡信号受体，广泛参与细胞程序性死亡、免疫稳态调控及肿瘤发生等生物学过程，本试剂盒适用于神经退行性疾病、自身免疫疾病及癌症机制等研究领域，帮助科研人员分析细胞凋亡相关通路中FAS/CD95的表达水平。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fas ELISA Kit; Apt1 ELISA Kit; Tnfrsf6 ELISA Kit; Tumor necrosis factor receptor superfamily member 6 ELISA Kit; Apo-1 antigen ELISA Kit; Apoptosis-mediating surface antigen FAS ELISA Kit; FASLG receptor ELISA Kit; CD antigen CD95 ELISA Kit
缩写：FAS
Uniprot No.：Q63199
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat FAS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 86-105 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 87-105 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of rat FAS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-105 | ||||
| EDTA plasma (n=4) | 95 | 93-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.5042.5322.5182.402252.3122.3452.3292.21312.51.8841.8791.8821.7666.251.1111.1331.1221.0063.120.5730.5860.5800.4641.560.3630.3740.3690.2530.780.2410.2550.2480.13200.1150.1170.116 | ||||||
货期：3-5 working days
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