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Human Protein FAM3C(FAM3C) ELISA kit

  • 中文名: 人FAM3C蛋白(FAM3C)酶联免疫试剂盒
货号: IPD-EL008228HU
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人FAM3C蛋白(FAM3C)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的FAM3C含量。FAM3C,也称为ILEI,是FAM3家族的一员,主要存在于分泌性上皮细胞中。它在视网膜层状形成、上皮间质转化和胰腺癌发生中发挥重要作用。研究机制上,FAM3C可能通过调节细胞分化和组织重塑影响细胞行为,其具体作用机制尚在研究中。试剂盒检测范围为1.56 ng/ml- 100 ng/ml,适用于体外培养细胞模型、动物实验组织样本或人类体液样本中FAM3C蛋白表达水平的动态监测,为探索其在肿瘤微环境调控、代谢性疾病机制或药物干预研究提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Fam3c ELISA Kit; FAM3C_HUMAN ELISA Kit; Family with sequence similarity 3 member C ELISA Kit; GS3786 ELISA Kit; ILEI ELISA Kit; Interleukin-like EMT inducer ELISA Kit; Predicted osteoblast protein ELISA Kit; Protein FAM3C ELISA Kit
  • 缩写:
    FAM3C
  • Uniprot No.:
    Q92520
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    1.56 ng/ml - 100 ng/ml
  • 灵敏度:
    0.39 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human FAM3C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    93


    Range %

    88-98


    1:2

    Average %

    96


    Range %

    92-101


    1:4

    Average %

    92


    Range %

    87-98


    1:8

    Average %

    97


    Range %

    94-101


  • 回收率:

    The recovery of human FAM3C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    98

    90-105


    EDTA plasma (n=4)

    93

    89-100


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    100

    1.941

    2.010

    1.976

    1.875


    50

    1.476

    1.570

    1.523

    1.422


    25

    1.073

    1.061

    1.067

    0.966


    12.5

    0.702

    0.751

    0.727

    0.626


    6.25

    0.404

    0.432

    0.418

    0.317


    3.12

    0.312

    0.322

    0.317

    0.216


    1.56

    0.201

    0.205

    0.203

    0.102


    0

    0.102

    0.099

    0.101




  • 货期:
    3-5 working days

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