首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠组织因子(TF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织培养上清液、组织匀浆、细胞裂解物样本中的F3含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，试剂盒适用于心血管疾病模型、肿瘤生物学研究、炎症相关实验等科研场景，通过检测不同样本类型中TF的动态变化，可深入探究其在疾病发生发展中的作用机制，或用于药物干预实验中生物标志物的效果评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：F3 ELISA Kit; Cf3 ELISA Kit; Tissue factor ELISA Kit; TF ELISA Kit; Coagulation factor III ELISA Kit; CD antigen CD142 ELISA Kit
缩写：F3
Uniprot No.：P42533
种属：Rattus norvegicus (Rat)
样本类型：serum, urine, cell culture supernates, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Blood Coagulation
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 91-106 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 93-108 | |||||
回收率：
| The recovery of rat TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 84-94 | ||||
| EDTA plasma (n=4) | 103 | 95-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.2972.2312.2642.08110001.5731.4711.5221.3395000.9990.9240.9620.7792500.6180.5930.6060.4231250.4050.4250.4150.23262.50.2930.3100.3020.11931.250.2490.2640.2570.07400.1770.1880.183 | ||||||
货期：3-5 working days
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