| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | RBP3; E2F-1; RBAP1; RBBP3 |
| WB Predicted band size | 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human E2F1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于E2F1抗体的示例文献摘要(虚构示例，仅供格式参考)：
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1. **"E2F1 regulates cell cycle progression via direct DNA binding in cancer cells"**
- Author: Johnson et al. (2015)
- 摘要：本研究使用E2F1抗体通过染色质免疫沉淀(ChIP)和Western blot技术，证实E2F1在G1/S期转换中直接结合靶基因启动子区域，并揭示其在乳腺癌细胞周期异常中的调控作用。
2. **"Overexpression of E2F1 correlates with poor prognosis in glioblastoma"**
- Author: Smith & Lee (2018)
- 摘要：通过免疫组化(IHC)和E2F1特异性抗体分析胶质母细胞瘤组织，发现E2F1蛋白高表达与患者生存期缩短显著相关，提示其作为潜在治疗靶点。
3. **"Post-translational modification of E2F1 impacts its stability and transcriptional activity"**
- Author: Chen et al. (2020)
- 摘要：利用E2F1抗体进行免疫共沉淀(Co-IP)实验，证明E2F1的磷酸化修饰通过泛素-蛋白酶体途径调控其蛋白稳定性，影响细胞凋亡与增殖平衡。
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注：以上为模拟摘要，实际文献需通过PubMed/Google Scholar检索关键词(如"E2F1 antibody application"或"E2F1 immunohistochemistry")。真实研究中，E2F1抗体常用于检测蛋白表达、定位及功能机制研究。
The E2F1 antibody is a crucial tool in molecular biology research, primarily used to study the E2F1 transcription factor—a key regulator of cell cycle progression and apoptosis. E2F1 belongs to the E2F family of proteins, which control the transition from G1 to S phase by regulating genes involved in DNA replication and cell division. Unlike other E2F members, E2F1 exhibits dual roles: it promotes cell proliferation under normal conditions but induces apoptosis in response to DNA damage or oncogenic stress, making it a context-dependent tumor suppressor or oncogene.
Researchers employ E2F1 antibodies in techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunohistochemistry (IHC) to analyze its expression, localization, and DNA-binding activity. These applications are vital for understanding its role in cancer biology, as E2F1 dysregulation is linked to tumor development, chemotherapy resistance, and metastatic potential in various cancers. The antibody’s specificity is critical, often validated using knockout cell lines or competitive peptides to ensure accurate detection of the ~60 kDa protein.
Commercial E2F1 antibodies are typically monoclonal or polyclonal, with selection depending on experimental needs. Recent studies also explore its non-canonical functions in metabolism, differentiation, and immune response, expanding its relevance beyond traditional cell cycle research. Proper antibody validation remains essential to avoid cross-reactivity with other E2F family members (e.g., E2F2. E2F3) and ensure research reproducibility.
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