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产品描述：人凝血因子XI(F11)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的F11含量。人凝血因子XI（F11）是一种丝氨酸蛋白酶原，由肝细胞和巨核细胞合成，通过激活凝血因子IX参与凝血过程。其缺陷症为常染色体隐性遗传性疾病，与F11基因突变相关，导致凝血功能障碍和出血倾向。研究其作用机制有助于开发新的抗血栓药物和治疗方法。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于体外模拟凝血微环境时细胞因子表达监测、抗凝药物对F11调控作用的评估，或心血管疾病模型中凝血相关蛋白网络的动态分析。为凝血机制基础研究、血栓性疾病动物模型验证及靶向治疗研究提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：coagulation factor XI ELISA Kit; Coagulation factor XIa light chain ELISA Kit; F11 ELISA Kit; FA11_HUMAN ELISA Kit; FXI ELISA Kit; MGC141891 ELISA Kit; Plasma thromboplastin antecedent ELISA Kit; Platelet coagulation factor XI ELISA Kit; PTA ELISA Kit
缩写：F11
Uniprot No.：P03951
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Blood Coagulation
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human F11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 85 | |||||
| Range % | 82-88 | ||||||
| 1:2000 | Average % | 110 | |||||
| Range % | 107-113 | ||||||
| 1:4000 | Average % | 91 | |||||
| Range % | 85-97 | ||||||
| 1:8000 | Average % | 92 | |||||
| Range % | 87-95 | ||||||
回收率：
| The recovery of human F11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 98-106 | |||||
| EDTA plasma (n=4) | 86 | 82-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected50002.7042.6812.6932.61725002.0922.1382.1152.03912501.3871.4051.3961.3206250.8450.8680.8570.7813120.5030.5380.5210.4451560.3010.2960.2990.223780.1760.1860.1810.10500.0770.0740.076 | |||||||
货期：3-5 working days
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