首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：猪凝血因子X (F10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F10含量。F10即凝血因子X，其在凝血级联反应中至关重要，激活后能促使凝血酶原转变为凝血酶，推动纤维蛋白原形成纤维蛋白，完成凝血过程。针对F10的研究聚焦于开发新型抗凝药物，抑制其活性来防治血栓性疾病。试剂盒检测范围为0.312 μg/mL-20 μg/mL，该产品适用于动物疾病模型研究（如凝血功能障碍、肝脏疾病）、营养代谢实验或药物毒理学评估等科研领域，为探究F10在猪凝血机制、止血调控及相关病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：F10
Uniprot No.：F1RN41
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.027 μg/mL-20 μg/mL
灵敏度：0.027 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Blood Coagulation
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig F10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 93 | |||||
| Range % | 87-105 | ||||||
| 1:10 | Average % | 96 | |||||
| Range % | 90-110 | ||||||
| 1:20 | Average % | 92 | |||||
| Range % | 89-99 | ||||||
| 1:40 | Average % | 95 | |||||
| Range % | 83-100 | ||||||
回收率：
| The recovery of pig F10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 88-106 | |||||
| EDTA plasma (n=4) | 96 | 89-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| µg/mlOD1OD2AverageCorrected 201.8271.8011.8141.675 6.671.6581.6721.6651.526 2.221.4211.4481.4351.296 0.740.9570.9740.9660.827 0.250.6420.6650.6540.515 0.0820.3890.3730.3810.242 0.0270.2410.2390.2400.101 00.1370.1410.139 | |||||||
货期：3-5 working days
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